Seed Germination Lab

In Kayleigh Magahan, Frederick Styles, and Sofia Dinges’ experiment of the Effects of Acidity on Radishes is similar to our experiment. In their experiment, they planted three radish seeds in three jars. In addition, they also used water as the neutral substance, while they used 5.6 pH and 4.5 pH solutions to figure out the effects of acidity on the radishes. They also measured the root as well. Although the base is not present in this experiment, the pH levels were the experimental variable, while the neutral substance was the controlled variable. (Effects of Acidity on Radishes -

The purpose of this experiment is to investigate whether the size of the bean will affect the height and growth rate of the bean stalk. According to Anthony Brach Ph.D., the larger seed size tends to grow slower because they require more nutrients than smaller seeds, however, larger seeds will often grow taller because they have a lot of nutrients stored in them. Mung beans, are a legume likely native to India. These bean plants require full sunlight or at least eight to 10 hours of sunlight daily. Mung beans can grow up to 36 inches tall and reaches maturity after 120 days. The goals of this experiment are to learn how to successfully

Just wanted to share with you the template for the Seed Germination Inquiry Lab Report {Template}. The general information filled in, all that is left is the remaining information (based on the experiment). Afterward, you should be complete with the lab report and can turn it in to the instructor.

The aim of this practical is to investigate the effects of gibberellic acid on the germination rates of two plant species. It was hypothesised from the above information that higher

This experiment was focused on the effect that salt water has on bean plants, as opposed to tapwater. Specifically, does watering a plant with saltwater, instead of tap water make plants grow healthier or faster? It was hypothesised that if a plant is watered with only salt water, then the plant will not grow as well as one watered with tap water. During this experiment, The quantitative data that was collected included the plant height and the amount of salt that accumulated on the surface of the soil. The qualitative data was the color of the plants, soil, salt clumps, and the appearance of the plants overall.

If more water is distributed to the seeds than the growth of the seeds will be limited.

To perform the experiment, three of 6-in diameter plant pots with water holes, moist soil, round radish seeds, and 3.5 liters of water in total used every other day in 0.5 liters for 14 days, were used. On November 5th, round radish seeds were planted in three identical 6-in diameter pots with various distances between the seeds. The distance between seeds for each pots were ½ inches (1.27cm), 2 inches (5.08cm), and 3 inches ( 7.62cm). Seeds were planted 1 inch deep under the soil and were constantly watered every other day with 500 ml paper cup, which was just enough to moist soil. All the pots were constantly located under the direct sunlight. A picture was taken inconsistently to keep track of any conditions that could affect the outcome.

Auxin, a hormone that influences stem elongation and controls the development of plant tissue where growth occurs, is a vital chemical that shows a positive correlation with shoot growth rate (Went 2001). According to the Journal of Experimental Botany, Auxin signaling in the shoot has to be exactly balanced in order to stimulate cell reproduction, therefore, too much or too little of the hormone may inhibit or halt plant progression (Gallavotti 2013). Gibberellin, or Gibberellic Acid, also plays an important role in plant growth, specifically targeting leaf growth and stem lengthening (Brian 2008).

The 20 wheat seedlings was the sample size used in each of the five treatments, see Table 1. Among those 10 unpaired t-tests two out of the 10, Test 5 (Shoot length 1XCM vs 4XCM) and Test 6 (Seedling biomass Water vs 1XCM) had actual p-values greater than 0.05 (see Table 1). The calculated t-values for the previously mentioned two t-tests were lower than the critical t-value, 2.024 (see Table 1). For shoot length (cm), the trend shows mean shoot length to increase with increasing concentration of nutrient treatment until 3XCM, where the mean and standard deviation is at its greatest (x̅= 27.945cm±5.605cm) and drops slightly at 4XCM (see Figure 1). The pattern of the mean biomass (g), increases up to treatment 2XCM (x̅=0.2275g±0.0488g) at which mean values begin to decline a slight degree afterwards (see Figure 2).

Each treatment number represented a specific treatment, treatment 1 was the control, treatment 2 was the N-deficient, treatment 3 was he P-deficient, treatment 4 was the K-deficient and treatment 5 was the water treatment. Starting from the bottom of the container there were several layers; folded paper towels, cotton, another layer of folded paper towels, 30 mL of the treatment, evenly spread 10 mL of rye seeds, folded paper towels and 20 mL of the same treatment. The containers were placed under the fluorescent light for two weeks. After two weeks, using the dissecting probe remove 20 plants from the middle, left and right corners from each growth container. The seed and root was removed using scissors, leaving the shoot behind. The shoot was then placed on the scale and weighed for shoot biomass, in milligrams, which was converted to grams by multiplying 1000, and the shoot length was recorded in centimeters. The shoot’s biomass and length were transferred to the Data Sheet and to a table on Excel. Two individual graphs, shoot’s biomass and length, were made on Excel after calculating the statistical data.

The process of seed germination is dependent on both environmental and internal, hormonal factors. Controlled by a number of mechanisms necessary for growth and development, seed germination rely on interactions between plant hormones, genes, and the environment it is surrounded by (Miransari and Smith, 2013). Just as germination is a complex, developmental phase, so is senescence. Involving both degenerative and nutrient recycling processes, senescence is the final stage of growth and development (Zhang and Zhou, 2012). A kind of programmed cell death, senescence can be induced by both environmental and internal factors (Zhang and Zhou, 2012).

The experiment can also conclude the extent that pH affects Indian Lotus growth. It may conclude that varying pH has a slight effect on Indian Lotus growth or even the ability to stop it from undergoing photosynthesis completely and kill the organism. The experiment is important in general to find how pH can affect Indian Lotus growth which can in turn affect other aspects such as the diet of locals or lead to even a decrease in Indian Lotus which is culturally significant to cultures and religions.

The seeds were surface sterilized with 0.1% Mercuric chloride for 5 minutes then washed with distiied water several times to remove chemicals. The seeds of Raphanus and Cucumber were spread on sterile filter paper in each petridish and soaked in aqueous root exudates of each weeds. Each weeds had three replicates with equal quantity of seeds in petridishes. The seeds are allowed to grow for 10 days at room temperature. The seeds treated with sterile distilled water was termed as control. The seeds treated with weeds aqueous root exudates of Ageratum conyzoides, Leucas aspera, Scoparia dulcis, Spilanthes acmella and Vernonia patula were termed respectively as T1, T2, T3, T4 and T5. During the experiment period, care was taken to add equal volume of root exudates in each petridish periodically. After 10 days the seedlings were harvested. Then the effect of root exudates on the seed germination and seedling growth were studied. The shoot and root length of the seedlings were measured in cm and recorded. Then the average shoot and root length were calculated. The result observed are presented in Tables and photo plates.

To find the concentration of acidity in a particular substance a pH test is completed. The term pH stands for potential hydrogen, measuring how acidic or basic a substance is. Acids which have a higher concentration of H+ ions have a pH of less than 7; however, bases which have a lower concentration of H+ ions have a pH greater than 7. The pH of a substance is essential to understanding whether organisms can or cannot survive in a certain environment. For example sweet potatoes optimal pH range for growing is between 5.0-5.5; however, alfalfa’s is between 6.5 to 7.0. These two different plants cannot survive as well in environments with different pH’s. One factor that changes the pH of soil is acid rain. Although normal rain has a pH of 5.6,acid rain has a pH between 4.2-4.4. As a result, acid rain increases the acidity in soil, harming plants.

There are three components required for germination, each tightly regulated by temperature, are the plant hormones, enzymes activity and moisture. There are two self-secreted plant hormones that antagonize each other to regulate germination, abscisic acid (ABA) is