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- O b. False How much of ethanol you need to prepare 25% (v/v) ethanol solution Select one: O a. 75 ml O b. 75 g O c. 25 ml Od. 25 g The function of buffers is to Select one: ea Resist changes in pH. Ob. Fx the pH of solution Oc Resist changes in confugate acid .concentration d. Resist changes in weakacid .concentration1- Using the pH 12 of the buffer and the pKa = 12.32. What is the molar ratio of A- to HA. 2- What is the molar amount of A- and HA needed to prepare a 0.2 M buffer? 3- How many grams of A- and HA are needed to prepare 40 mal of the buffer? The molar mass of HA is 142g/mol The molar mass for A- is 380.1g/molAn appropriate biochemical buffer should have charges in both its conjugate acid and conjugate base form. This avoids the presence of a neutral form of the buffer that might be able slowly leak into cells. Does unmodified dimethylpiperazine meet this criterion when buffering at pH 8? O Yes No What about buffering at pH 4? D Yes No
- . An acetate/acetic acid (pKa=4.75) buffer of 500 mL includes 0.1 mol of acetate and 0.5 mol of acetic acid. 2.0 M NaOH was added to the buffer to get the pH to 4.70. What volume of NaOH was added to the system?Be sure to answer all parts. Tris [tris(hydroxymethyl)aminomethane] is a common buffer for studying biological systems. (K, = 5.01 x 109 and pk, 8.3) %3D %3D (a) Calculate the pH of the Tris buffer after mixing 10.5 mL of 0.20 M HCI solution with 25.0 mL of 0.10 M Tris. 7.06 (b) This buffer was used to study an enzyme-catalyzed reaction. As a result of the reaction, 0.00020 mol of H* was produced. What is the pH of the buffer at the end of the reaction? (c) What would be the final pH if no buffer were present?gnment.doc?_&d x True and False Assignment.doX true or false Any condition tha x content/enforced/21069983-BL_SCI_SNC2D1-7A_993734_2122Sem3/Activity3%20Assignm BIU A - A - 2. Identify the following bonds as ionic or covalent. a) NaF b) MgCl2 c) C6H1206 d) NO2 e) CaF2 f) LiF 3. Complete the following table. Electron transfer Lewis diagram Atoms to be bonded Li & CI !!!
- NaOH.2L X.02mol/L = .004 mol, H3PO4.8L X.15 mol/L = .12 mol H₂PO4 HPO4² + H+ .108 mol start A. If 200 mls of 0.02 M NaoH is added to 800 mls of 0.15 M phosphoric acid buffer at pH 8.2, what is the resultant pH? (pK1 = 2.1, Pk2=7.2, pk3=12.2) add .004 mol OH- end .012mol -.004 .008 mol pH= pka + log [A-]/[HA] = 7.2 + log.112/.008 +.004 .112mol pH=8.35 B. If the same amount of NaOH in part A is added to 800 mls of water, what is the resulting pH? .004mol/1L =.004 [OH-] pOH = 2.4, pH -11.67. · In the space provided, sketch a titration curve (pH vs H+ ions dissociated) of a phosphate buffer shown below. The pKa of H2PO4 is 7.0. H,PO, НРО, + 9. 8. 7 pH 3 1.0 0.5 H+ dissociated --> b. What is the effective buffering range of this acid? At what pH will you have 25% of the buffer in the form of H2PO4? с.Size Size Crude Anion Cation 2) Exclusion Exclusion Lysate Exchange Exchange 1 2 Total Protein Concentration 15.2 6.6 2 3.75 4.7 (mg/mL) Final Sample Volume 60 30 20 4 3 (mL) Enzyme Specific Activity 0.43 1.7 7.25 18.3 12.5 (units/mgprotein) Based on your protein purification sample data, in which of the purification steps in your protocol did you effectively purify your enzyme? Select all that apply. a) anion exchange correct b) cation exchange correct c) size exclusion 1 correct d) size exclusion 2
- Part I. For each of the following four questions: calculate and describe how the requested solution would be made. Please show all of your calculations. In all cases the diluent or solvent will be water. Also, assume the smallest volume you can accurately pipette is 1ul. (CaClh: Molecular weight = 111.0 - NaCl: Molecular weight= 58.44) 1. From a 3M CaCh stock solution, you need to make 600 ml of 9 mM CaClh. Indicate how you would make it. 2. You have a 20ul sample of DNA that you want to run in a gel. You are given 5X track dye; how much track dye do you add to your sample so that the track dye concentration in the sample is 1X? 3. You need to make 6 liters of 20% NaCl solution. Indicate how you wvould make it firom powdered NACI.More ratios. Through the use of nuclear magnetic resonance spectroscopy, it is possible to determine the ratio between the protonated and deprotonated forms of buffers. (a) Suppose the ratio of [ A- ]A I to [HA] is determined to be 0.1 for a buffer with pKar6.0.pKa = 6.0. What is the pH? (b) For a different buffer, 91974 suppose the ratio of [ A- ]lA J to [HA] is determined to be 0.1 and the pHpH is 7.0. In this case, what is the pKapKa of the buffer? (c) For another buffer with pKa=7.5PKa = 7.5 at pH 8.0pH 8.0, what is the expected ratio of [ A- ][A ] to [HA]? doYou need to make a protein buffer of: . • • 100 mM NaCl 25 mM Tris 8 5% w/v glycerol • 2 mM DTT Your stock solutions are: 5 M NaCl • 2 M Tris 8 • 70% w/v glycerol • DTT @ 154.25 g/mol How would you make a 1L protein buffer Solution? Show your work and describe.
