1. For this mixture of polyaromatic hydrocarbons (PAH's), which LC column stationary phase would be expected to give the best separation? C18 Phenyl Cyano www m

Fundamentals Of Analytical Chemistry
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ISBN:9781285640686
Author:Skoog
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Chapter33: High-performance Liquid Chromatography
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Problem 33.15QAP
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Give answer all questions with explanation and answer no. Please
1. For this mixture of polyaromatic hydrocarbons (PAH's), which LC column stationary phase would be
expected to give the best separation?
C18
Phenyl
Cyano
Explain the basis of your choice.
2. Using a C18 HPLC column and a mobile phase with 10% acetonitrile, the retention times of a mixture
are 2.35 minutes. 3.78 minutes and 5.72 minutes. If the acetonitrile content is increased to 15%, would
you expect these retention times to increase or decrease? Explain your answer.
3. Size exclusion chromatography (SEC) relies on separation associated with how far an analyte
penetrates into the pores in the stationary phase. It is used to separate species with molecular weights
much greater than 1000 Daltons. How does the retention time vary with molecular weight in an SEC
separation of molecules with similar chemical structures?
Transcribed Image Text:1. For this mixture of polyaromatic hydrocarbons (PAH's), which LC column stationary phase would be expected to give the best separation? C18 Phenyl Cyano Explain the basis of your choice. 2. Using a C18 HPLC column and a mobile phase with 10% acetonitrile, the retention times of a mixture are 2.35 minutes. 3.78 minutes and 5.72 minutes. If the acetonitrile content is increased to 15%, would you expect these retention times to increase or decrease? Explain your answer. 3. Size exclusion chromatography (SEC) relies on separation associated with how far an analyte penetrates into the pores in the stationary phase. It is used to separate species with molecular weights much greater than 1000 Daltons. How does the retention time vary with molecular weight in an SEC separation of molecules with similar chemical structures?
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