A B Figure 1 The postgraduate student, Vanessa, cloned her gene of interest into two different vectors; pUC18 and pZERO®-1, and transformed the competent cells with the recombinant vectors. After that, she plated them on the antibiotic plates and incubated her samples for 18 hours. The next day, she realized that the plates were not labelled. (i) Based on the observation above, can you identify which plate is carrying the recombinant pUC18 and pZErOⓇ-1, respectively? (ii) Why are there small colonies surrounding the big colonies in Figure 1 (A)? Explain your answer in detail. (iii) Do you think all the colonies present on the plates in Figure 1 (B) contain the gene of interest? Describe the selection procedures for recombinant pZERO®-1.
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
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