A lab trainer instructed a new lab recruit to freeze down cells from a tissue culture flask to save for future use. After verifying that the flask of adherent cells was confluent, and contained enough cells to freeze down, the lab trainer left the recruit to go through the freezedown protocol. A few days later, the trainer thawed out the cryovials of cells that the recruit put away, and discovered that there were very few viable cells. Assuming that the lab trainer did everything correctly, answer the following: What are two possible mistakes made that explains why there are so few cells? Explain why those mistakes had the effect they did.

A lab trainer instructed a new lab recruit to freeze down cells from a tissue culture flask to save for future use. After verifying that the flask of adherent cells was confluent, and contained enough cells to freeze down, the lab trainer left the recruit to go through the freezedown protocol. A few days later, the trainer thawed out the cryovials of cells that the recruit put away, and discovered that there were very few viable cells. Assuming that the lab trainer did everything correctly, answer the following: What are two possible mistakes made that explains why there are so few cells? Explain why those mistakes had the effect they did.

Basic Clinical Laboratory Techniques 6E

6th Edition

ISBN:9781133893943

Author:ESTRIDGE

Publisher:ESTRIDGE

Chapter2: Basic Hematology

Section2.12: Reticulocyte Count

Problem 5RQ

See similar textbooks