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A researcher wants to try and express a protein, creatively named Protein Cancerdeath, in Escherichia coli bacterial cells. Previous work has shown that humans Who have the gene that codes for Protein Cancerdeath have much lower cancer rates than those of the average human population. The gene that codes for Protein Cancerdeath has been sequenced, and the researcher inserts this sequence into a plasmid that is placed in E. col bacterial cells, along with an affinity tag to faciliate protein purification. The protein is expressed in E. coli, purified from the bacterial cells, and the affinity tag is cleaved off. However, when using this E. coli-expressed Protein Cancerdeath protein in further studies, not only does it not yield any effectiveness against cancer, but also it is twice the mass of the Protein Cancerdeath that was isolated from actual humans. What is the most likely mistake the researcher made? Choose one: O A. The plasmid containing the gene for Protein Cancerdeath was not succesfully inserted into the E. coli cells, such that the appropriate protein was never expressed to begin with. O B. The researcher inserted the entire DNA sequence/gene for Protein Cancerdeath into E. coli, instead of using the protein sequence to determine the mature MRNA sequence, and then reverse engineering the gene to insert into E. coli. O C. The purification process, in which the protein was purified from E. coli cell extract, was not done properly. and there were too many contaminating species that interfered with results. O D. E coliwas not able to add the sugar groups that humans add to the Protein Cancerdeath (a post-translational modification). These sugar groups would account for 1% of the overall mass of the protein in humans.

A researcher wants to try and express a protein, creatively named Protein Cancerdeath, in Escherichia coli bacterial cells. Previous work has shown that humans Who have the gene that codes for Protein Cancerdeath have much lower cancer rates than those of the average human population. The gene that codes for Protein Cancerdeath has been sequenced, and the researcher inserts this sequence into a plasmid that is placed in E. col bacterial cells, along with an affinity tag to faciliate protein purification. The protein is expressed in E. coli, purified from the bacterial cells, and the affinity tag is cleaved off. However, when using this E. coli-expressed Protein Cancerdeath protein in further studies, not only does it not yield any effectiveness against cancer, but also it is twice the mass of the Protein Cancerdeath that was isolated from actual humans. What is the most likely mistake the researcher made? Choose one: O A. The plasmid containing the gene for Protein Cancerdeath was not succesfully inserted into the E. coli cells, such that the appropriate protein was never expressed to begin with. O B. The researcher inserted the entire DNA sequence/gene for Protein Cancerdeath into E. coli, instead of using the protein sequence to determine the mature MRNA sequence, and then reverse engineering the gene to insert into E. coli. O C. The purification process, in which the protein was purified from E. coli cell extract, was not done properly. and there were too many contaminating species that interfered with results. O D. E coliwas not able to add the sugar groups that humans add to the Protein Cancerdeath (a post-translational modification). These sugar groups would account for 1% of the overall mass of the protein in humans.

Chemistry for Today: General, Organic, and Biochemistry
Chemistry for Today: General, Organic, and Biochemistry
9th Edition
ISBN:9781305960060
Author:Spencer L. Seager, Michael R. Slabaugh, Maren S. Hansen
Publisher:Spencer L. Seager, Michael R. Slabaugh, Maren S. Hansen
Chapter21: Nucleic Acids And Protein Synthesis
Section: Chapter Questions
Problem 21.59E
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A researcher wants to try and express a protein, creatively named Protein Cancerdeath, in Escherichia coli bacterial cells. Previous work has shown that humans Who have the gene that codes for Protein Cancerdeath have much lower cancer rates than those of the average human population. The gene that codes for Protein Cancerdeath has been sequenced, and the researcher inserts this sequence into a plasmid that is placed in E. col bacterial cells, along with an affinity tag to faciliate protein purification. The protein is expressed in E. coli, purified from the bacterial cells, and the affinity tag is cleaved off. However, when using this E. coli-expressed Protein Cancerdeath protein in further studies, not only does it not yield any effectiveness against cancer, but also it is twice the mass of the Protein Cancerdeath that was isolated from actual humans. What is the most likely mistake the researcher made? Choose one: O A. The plasmid containing the gene for Protein Cancerdeath was not succesfully inserted into the E. coli cells, such that the appropriate protein was never expressed to begin with. O B. The researcher inserted the entire DNA sequence/gene for Protein Cancerdeath into E. coli, instead of using the protein sequence to determine the mature MRNA sequence, and then reverse engineering the gene to insert into E. coli. O C. The purification process, in which the protein was purified from E. coli cell extract, was not done properly. and there were too many contaminating species that interfered with results. O D. E coliwas not able to add the sugar groups that humans add to the Protein Cancerdeath (a post-translational modification). These sugar groups would account for 1% of the overall mass of the protein in humans.
Transcribed Image Text:A researcher wants to try and express a protein, creatively named Protein Cancerdeath, in Escherichia coli bacterial cells. Previous work has shown that humans Who have the gene that codes for Protein Cancerdeath have much lower cancer rates than those of the average human population. The gene that codes for Protein Cancerdeath has been sequenced, and the researcher inserts this sequence into a plasmid that is placed in E. col bacterial cells, along with an affinity tag to faciliate protein purification. The protein is expressed in E. coli, purified from the bacterial cells, and the affinity tag is cleaved off. However, when using this E. coli-expressed Protein Cancerdeath protein in further studies, not only does it not yield any effectiveness against cancer, but also it is twice the mass of the Protein Cancerdeath that was isolated from actual humans. What is the most likely mistake the researcher made? Choose one: O A. The plasmid containing the gene for Protein Cancerdeath was not succesfully inserted into the E. coli cells, such that the appropriate protein was never expressed to begin with. O B. The researcher inserted the entire DNA sequence/gene for Protein Cancerdeath into E. coli, instead of using the protein sequence to determine the mature MRNA sequence, and then reverse engineering the gene to insert into E. coli. O C. The purification process, in which the protein was purified from E. coli cell extract, was not done properly. and there were too many contaminating species that interfered with results. O D. E coliwas not able to add the sugar groups that humans add to the Protein Cancerdeath (a post-translational modification). These sugar groups would account for 1% of the overall mass of the protein in humans.
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