Considering the principle of the neutral red bioassay, describe “in details” the expected appearance of the check/buccal cells if exposed to a toxic substance such as cigarette ash or possibly any other type of toxic substance. Illustrate the expected finding.g
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Considering the principle of the neutral red bioassay, describe “in details” the expected appearance of the check/buccal cells if exposed to a toxic substance such as cigarette ash or possibly any other type of toxic substance. Illustrate the expected finding.g
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- Cancer stem cells (CSCs) are cancer cells (found within tumors or hematological cancers) that possess characteristics associated with normal stem cells, specifically the ability to give rise to all cell types found in a particular cancer sample. There are many biomedical engineering based approaches to detect CSCs. 1) What is the importance and advanatge of detecting CSCs? 2) What kind of systems have been developed to detect CSCs? Describe by giving examples. Please explain in detail with your own words.Explain the limitations of screens for mutants withmorphological phenotypes, and discuss how sensitizedmodifier screens can help overcome these issues.INTERPRETATION OF RESULIS NEGATIVE: Two lines appear. One colored line should be in the control region (C), and another apparent colored or faded color line adjacent should be in the test region (T). This negative result indicates that the drug concentration is below the detectable level. POSITIVE: One colored line appears in the control region (C). No line appears in the test region (T). This positive result indicates that the drug concentration is above the detectable level. INVALID: Control line fails to appear. Insufficient specimen volume or incorrect procedural techniques are the most likely reasons for control line failure. Review the procedure and repeat the test using a new test panel. If the problem persists, discontinue using the lot immediately and contact your local distrībutor. READING OF RESULTS INTERPRETATION SONTROL TEST SAMPLE
- Briefly explain this statement -"Therapeutic drug monitoring of Warfarin" Please explain at your own words(500-600 words appropriately).A. Prepare a diagram with labels showing the similarities and differences among the four major types of ELISA based on their capture and detection systems. A1. Give at least one clinical application for each type of ELISA. B. What is the purpose of adding horseradish peroxidase, alkaline phosphatase, and their respective substrates?Give typing answer with explanation and conclusion 8. Considering the principle of the Neutral Red bioassay, describe, in details, the expected appearance of the cheek/buccal cells if exposed to a toxic substance such as cigarette ash or possibly any other type of toxic substance.
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- State the principle of the antiglobulin test. Differentiate monoclonal from polyclonal and monospecific from polyspecific antihuman globulin (AHG) reagents.1) A) List 15 drugs (monoclonal antibodies can be used) used clinically to treat cancer in humans. These targets must be signal transduction pathway components. B) For each drug, list the specific protein targeted. C) For each drug, describe the efficacy of treatment (i.e. what is the success rate in life extension) as well as appropriate cost of treatment whether it be per round or an average annual cost.Distinguish between What is known of CD105 (endoglin) as an hepatcellular carcinoma marker and it’s potential as a drug target. Discuss: - Is anything known about its biology? E.g does it have known ligands and normal functions? - if you were to target it would you just use it to get growth inhibiting compound into the new blood vessels or is CD105 required for neovascularization?