Each of the three different Hfr strains in the table below (A, B and C) arose independently and possess the same markers. Each of the three Hfr strains was separately mixed with the same F strain carrying the alternative markers. Conjugation was interrupted at various times after mixing over a period of 95 minutes. The bacteria were then plated on the appropriate media and recipients scored for various Hfr markers. The times (in minutes after mixing) at which recombinants carrying particular donor markers first appear, and therefore the times at which these first enter the F- cell and then integrate into the recipient genome, are shown below: Donor markers Time of appearance in minutes Strain A Strain B Strain C his+ 6.5 68.5 29.5 gal+ 30.0 46.0 52.0 pro+ 34.5 41.5 56.5 met+ 58.0 20.0 76.0 mtl+ 67.0 11.0 85.0 xyl+ 68.5 9.5 86.5 mal+ 73.0 5.0 3.0 ser+ 81.5 86.5 11.5 tyr+ 88.0 78.5 19.5 Draw the genome of each strain showing the location of the markers, the location of F, and the direction of transfer during conjugation.
Each of the three different Hfr strains in the table below (A, B and C) arose independently and possess the same markers. Each of the three Hfr strains was separately mixed with the same F strain carrying the alternative markers. Conjugation was interrupted at various times after mixing over a period of 95 minutes. The bacteria were then plated on the appropriate media and recipients scored for various Hfr markers. The times (in minutes after mixing) at which recombinants carrying particular donor markers first appear, and therefore the times at which these first enter the F- cell and then integrate into the recipient genome, are shown below: Donor markers Time of appearance in minutes Strain A Strain B Strain C his+ 6.5 68.5 29.5 gal+ 30.0 46.0 52.0 pro+ 34.5 41.5 56.5 met+ 58.0 20.0 76.0 mtl+ 67.0 11.0 85.0 xyl+ 68.5 9.5 86.5 mal+ 73.0 5.0 3.0 ser+ 81.5 86.5 11.5 tyr+ 88.0 78.5 19.5 Draw the genome of each strain showing the location of the markers, the location of F, and the direction of transfer during conjugation.
Chapter10: Reconstitution Of Powdered Drugs
Section: Chapter Questions
Problem 33SST
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Transcribed Image Text:Each of the three different Hfr strains in the table below (A, B and C) arose independently
and possess the same markers. Each of the three Hfr strains was separately mixed with the
same F strain carrying the alternative markers. Conjugation was interrupted at various times
after mixing over a period of 95 minutes. The bacteria were then plated on the appropriate
media and recipients scored for various Hfr markers. The times (in minutes after mixing) at
which recombinants carrying particular donor markers first appear, and therefore the times at
which these first enter the F- cell and then integrate into the recipient genome, are shown
below:
Donor
markers
Time of appearance in minutes
Strain A
Strain B
Strain C
his+
6.5
68.5
29.5
gal+
30.0
46.0
52.0
pro+
34.5
41.5
56.5
met+
58.0
20.0
76.0
mtl+
67.0
11.0
85.0
xyl+
68.5
9.5
86.5
mal+
73.0
5.0
3.0
ser+
81.5
86.5
11.5
tyr+
88.0
78.5
19.5
Draw the genome of each strain showing the location of the markers, the location of F, and
the direction of transfer during conjugation.
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