Identify the method of sterilization/ chemical agent described At 15psi, the temperature reaches 121 degree Celsius requiring only 15 to 20 minutes to sterilize the material. The material to be sterilized is exposed to live steam for 30 minutes for 3 consecutive days.
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Identify the method of sterilization/ chemical agent described
- At 15psi, the temperature reaches 121 degree Celsius requiring only 15 to 20 minutes to sterilize the material.
- The material to be sterilized is exposed to live steam for 30 minutes for 3 consecutive days.
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- Disinfectants and Sterilization Procedures Complete the table by putting (+) for growth of microorganisms or (–) absence of growth of microorganisms. Disinfectant Concentration Growth after time of exposure (minutes) Control 5 minutes 10 minutes 15 minutes Sodium hypochlorite 5% Sodium hypochlorite 0.05% Alcohol 70% Alcohol 40% Iodophor (Betadine) 10% solution Mouthwash Cetylpyridinium Chloride - 0.075% Sodium Fluoride - 0.05%In sterilization, which among the supplies, instruments, glassware, etc. under the list of materials can be sterilized using either or both equipment below? List them down. For "autoclaving" For Dry heat oven Can be sterilized only sterilization with either Materials: 200-ml Erlenmeyer flask Stove 500-ml Erlenmeyer flask Autoclave 10-ml graduated cylinder Analytical balance 100-ml graduated cylinder pH meter Spatula Stirring rod 100-ml beaker Test tubes Distilled water Petri dish Stirring rod Alcohol lamp Glass dropperOther than the application of heat, name other methods of sterilization. Define the methods and give examples of materials sterilized in each method.
- Match the most appropriate method of sterilisation for the following items. 10 mL vitamin solution 5 mL antibiotic solution Petri dishes 1 L of water 1 L of nutrient broth medium Talcum powder Charcoal powder [Choose ] [Choose ] Gamma radiation Autoclave Dry heat oven Filtration [Choose ] [Choose ] [Choose ] [Choose ] [Choose ]What is the physical appearance (solid, liquid, semi-solid) of following media:Agar deep tubeOven sterilization at 121˚C was performed on glassware for 15 minutes only. Effect: Rationale:
- List 5 other methods of sterilization and describe the principle/s and when each method is used. What are the requirements to support microbial growth?What is the physical appearance (solid, liquid, semi-solid) of following media: agar plateMatch the description to the correct medium This medium is enrichment for fastidious [Choose ] bacterial pathogens and differential based on hemolysis [Choose ] This medium is selective based on Tryptic Soy Agar, TSA salt/sodium chloride tolerance at 7.5 % Sabouraud Dextrose Agar, "Sab" agar weight/volume and differential based on mannitol fermentation Mannitol Salt Agar, "MSA" MacConkey Agar, "Mac" agar This medium is selective based on the ability to grow in bile salts and crystal violet and is EMB Eosin Methylene Blue Agar differetial based on lactose fermentation Blood Agar, "BA" this medium is all purpose and can grow a [Choose ] wide range of bacteria and fungi
- Alternative name for Moist-heat sterilization Select one: Oven sterilization Autoclaving Ionizing radiationGive two reasons for heating the slide after the smear is air dried?You are given reference temperature 121 °C, Z value 10 °C, F0 15 min for killing Clostridium botulinum in commercial sterilization. Calculate F value when sterilized at 131 °C in canning processing.