Match the most appropriate method of sterilisation for the following items. 10 mL vitamin solution 5 mL antibiotic solution Petri dishes 1 L of water 1 L of nutrient broth medium Talcum powder Charcoal powder [Choose ] [Choose ] Gamma radiation Autoclave Dry heat oven Filtration [Choose ] [Choose ] [Choose ] [Choose ] [Choose ] >
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- Match the description to the correct medium This medium is enrichment for fastidious [Choose ] bacterial pathogens and differential based on hemolysis [Choose ] This medium is selective based on Tryptic Soy Agar, TSA salt/sodium chloride tolerance at 7.5 % Sabouraud Dextrose Agar, "Sab" agar weight/volume and differential based on mannitol fermentation Mannitol Salt Agar, "MSA" MacConkey Agar, "Mac" agar This medium is selective based on the ability to grow in bile salts and crystal violet and is EMB Eosin Methylene Blue Agar differetial based on lactose fermentation Blood Agar, "BA" this medium is all purpose and can grow a [Choose ] wide range of bacteria and fungiMatch the physical and chemical methods of control with its use, mode of action etc. Purify H₂0 Sterilize catheters, Petri plates, syringes, sutures placed in plastic wraps Sporocidal antiseptic Their mode of action is lowering the surface tension of water Antiseptic that kills anaerobic bacteria It is use to preserve cadavers, tissues and organs Isopropilic is the most germicidal Standard of disinfectants Choose... Choose... Choose... Choose... Choose... Choose... Choose... Choose... ◆ ◆ → →Examine the given sets of table below. Which disinfectant is the MOST recommended? Support and Justify your answer. Table 2. Phenol Coefficient of Test Disinfectant Name of disinfectant Growth after 5 minutes Dettol Phenol Name of disinfectant Z-germicSide Phenol Dilution 1:100 1:125 1:150 1:175 1:200 1:95 1:100 1:105 1:110 1:115 Dilution 1:100 1:125 1:150 1:175 1:200 1:95 1:100 1:105 1:110 1:115 - + + + + * + Growth after 5 mins + + + Growth after 10 minutes + + + + Growth after 10 minutes + + * Growth after 15 minutes + + + + + Growth after 15 mins + P .. + 9. + + +
- Define disinfection. Compare/contrast it with sterilization, antisepsis and bacteriostasis. Give the modes of action of the different antiseptics/disinfectants used in the activity (5% sodium hypochlorite (bleach), 0.05% sodium hypochlorite (bleach), 70% alcohol, 40% alcohol, Iodophor (Betadine), and Mouthwash)Item 1. Describe the BEST method of sterilization or treatment to reduce pathogens to safe level for each of these 5 items. 2. Explain why this is the best method and why it is BETTER than other possible methods. (Specific treatments were mentioned in lecture for each item.) Plastic milk containers 1. Preserving low-acid vegetables to kill Clostridium botulinum endospores. 2. Liquids contaminated with prions (list BOTH methods of sterilization discussed in lecture) 3. Skin 4. Media with heat sensitive nutrients 5.Preservatives are added to the following pharmaceutical products EXCEPT Select one: Ophthalmic solutions Injections in eye tissues Non-sterile Ointments Multi-dose sterile products
- Examine the given sets of table below. Which disinfectant is the MOST recommended? Support and Justify your answer. Table 2. Phenol Coefficient of Test Disinfectant Name of disinfectant Growth after 5 minutes Dettol Phenol Name of disinfectant Z-germicSide Phenol Name of disinfectant Jik Phenol KEY:- = No growth, + = Growth Dilution 1:100 1:125 1:150 1:175 1:200 1:95 1:100 1:105 1:110 1:115 Dilution 1:100 1:125 1:150 1:175 1:200 1:95 1:100 1:105 1:110 1:115 Dilution 1:100 1:125 1:150 1:175 1:200 1:95 1:100 1:105 1:110 1:115 + + + + + + + + Growth after 5 mins + + + + + + + Growth after 5 mins + + + + + + Growth after 10 minutes + + + + + Growth after 10 minutes + + + + + Growth after 10 mins + - + + + + + + Growth after 15 minutes + + + + + + Growth after 15 mins - + + + + + Growth after 15 mins + + + + - + + + +Identify the method of sterilization/ chemical agent described At 15psi, the temperature reaches 121 degree Celsius requiring only 15 to 20 minutes to sterilize the material. The material to be sterilized is exposed to live steam for 30 minutes for 3 consecutive days.G When is it appropriate for gloves X + rface/defaultui/player/modern.html?configuration=&preventRightClick=False&cc=en-US&ieCompatibilityMod... 2 Exit rientation to Infection Prevention Which of the following are proper disinfection steps? Check all the apply. O Remove organic matter O Disinfect only O Follow suggested contact time O Mix cleaning products to make the disinfectant more powerful O Disinfect O Disregard the product labels SUBMIT ENG IN
- Disinfectants and Sterilization Procedures Complete the table by putting (+) for growth of microorganisms or (–) absence of growth of microorganisms. Disinfectant Concentration Growth after time of exposure (minutes) Control 5 minutes 10 minutes 15 minutes Sodium hypochlorite 5% Sodium hypochlorite 0.05% Alcohol 70% Alcohol 40% Iodophor (Betadine) 10% solution Mouthwash Cetylpyridinium Chloride - 0.075% Sodium Fluoride - 0.05%1. Write briefly about Metabolism of drugs ( just 5 lines ) 2. Write briefly about Polyethylene glycol ( just 3 lines ) 3. The levigating agent , explain about its role in Suppositories manufacturing 4. Write in details about types of gel 5. Write in details about fusion method in ointment preparationWhat is the physical appearance (solid, liquid, semi-solid) of following media:Agar deep tube