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What is the reason for using blind tube in spectrophotometric and calorimetric studies?
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- Why is it important to use standards or to develop a standard curve in spectrophotometry?Why is it important that the standard curve you create in biological analyses with spectrophotometry is measured using specialty cuvettes?Define each of the following terms: A) What is resolution and how is resolution related to the wavelength of light used to illuminate the sample? B) What is the magnification of the specimen if you are using a 40x objective and a 10x eyepiece? C) How is the numerical aperture (NA) of a lens related to its ability to gather light from a specimen?
- What are the light sources used in UV-vis spectrophotometry.Why is the wavelength of the light that is released in spectrophotometric analysis longer than the wavelength of the light that is used to activate a fluorescent molecule?Explain the principle of the isoelectric focusing electrophoresis method and what it is used for.
- Here are the materials and method for the basic spectrophotometer experiment Materials:1. Paper template2. Scissor3. Light source (i.e. torchlight, portable light)4. Paperboard (black colour)5. Unused compact disc (CD)6. Camera detector (i.e. webcam, laptop, mobile phone) Methods: 1. Make a foldable paper spectrophotometer using the paper template and followthe instruction as in https://publiclab.org/sites/default/files/8.5x11minispec3.8.pdf2. Then, mounted the paper spectrophotometer to the camera detector.3. Download any freely available spectral analyzer.4. Test and observe the wavelength with and without the presence of a light source.5. Finally, observe the wavelength with a different transparent colour sheets (you canchoose any colour). Troubleshooting/ problem(s) encountered for basic spectrophotometer experiment.What are the underlying physical principles of paper chromatography? What is spectrophotometry, the essence of it?The following image is a scheme for serial dilutions prepared for spectrophotometric analysis. If the stock solution concentration is 0.05 % (v/v) can you calculate the other tube’s concentrations in % v/v? I've used this with direct dilutions, how would I use this on serial dilutions?