Q: what is a purpose statement for a paper?
A: A research paper basically is an essay in which it is explainy about what has been learned after…
Q: p between absorbance (A) and transmittance (T) for the basic spectrophotometer technique.
A: A method to measure how much a chemical substance absorbs light by measuring the intensity of light…
Q: How does a contaminant such as salt affect the absorption of light by a spectrophotometric sample?
A: Spectrophotometry is a method in which the absorption or transmission properties of a material is…
Q: Transillumination is used clinically for the detection of:
A: Transillumination is an examination that is done with the help of a strong light shining on a…
Q: How can a UV-Vis spectrophotometer be applicable in determining the absorbance/concentration of a…
A: A spectrophotometer is an instrument that is used to measure the intensity of light absorbed by the…
Q: What are the pros and cons of using the TCE stain-free method versus a traditional gel staining…
A: PAGE (polyacrylamide gel electrophoresis) is a well-known method for separating, detecting, and…
Q: What are the differences among native gel electrophoresis, SDS-PAGE, isoelectric focusing,…
A: The separation techniques are essential to identify and isolate biomolecules for further biological…
Q: WHAT ARE THE Steps in spectrophotometer
A: -Spectrophotometer is an optical instrument which is used to measure the concentration of a known…
Q: What is the meaning of the different values given engraved on each objective?
A: Microscopes are used in viewing microscopic organisms, including bacteria, parasites, etc., and also…
Q: What are the light sources used in UV-vis spectrophotometry
A: Spectrophotometer is an instrument used for measuring the concentration of solutes in solution by…
Q: What is the principle of Giemsa staining and components of Giemsa stain?
A: The cells are the basic structural and fundamental unit of the living system. It consists of…
Q: NMR is considered a low-sensitivity spectroscopic technique. What is the physical basis of NMR's…
A: Nuclear magnetic resonance is a natural phenomenon during which nuclei during a strong constant…
Q: When it comes to simple stains, why is time so important? And what is the purpose of rinsing the…
A: Microbiology is a hugely important field in both basic and applied research. New and fascinating…
Q: Please make comments on the clinical potential of Functional Near Infrared Spectroscopy (fNIRS)?…
A: Functional Near-Infrared Spectroscopy (fNIRS) is a technique of non-invasive brain imaging that…
Q: What are the most commonly used methods in staining? why?
A: Introduction Staining is a technique for enhancing contrast in material, usually on a tiny scale.…
Q: Describe how simple staining and fluorescence staining are similar and how they are different. What…
A: Microscopy is a technical field for the use of microscopes to visualize objects and locations of…
Q: What is shown? Why did we use the slide? What is the arrow pointing to? What did we use it for? What…
A: Microscope is a instrument which is used to visualise the specimen that can't be seen by naked eyes…
Q: Why is a blank used in the spectrophotometer before the actual pigment is tested?
A: spectrophotometer is a device used in spectral analytical techniques like UV spectroscopy , in which…
Q: If a circular object or specimen extends halfway across the scanning field, what isits diameter in…
A: Field of view is defined as the area of the slide that is seen when an observer looks through a…
Q: How can you tell if the current or the gel electrophoresis is already running and what are the…
A: Gel electrophoresis is the process where DNA, RNA, and protein fragments get separated depending on…
Q: At what wavelengths should the absorbances be read to examine the purity of a DNA sample with a…
A: A spectrophotometer is a tool that measures the number of photons (light energy) absorbed after…
Q: What are the limitations of UV Visible spectroscopy and fluorescence spectroscopy? Include reference…
A: Spectrophotometric analysis has been widely used in biomedical science to determine the…
Q: Can colorless samples be analyzed using a UV-Vis spectrophotometer? Yes or No or Maybe?
A: UV-Vis Spectroscopy It is defined as a quantitative technique that is used to identify the…
Q: What are 2 advantages to using absorbance values (from a Spectrophotometer) over a spread plate and…
A: Introduction: Spectrophotometry is a method utilized for the evaluation and portrayal of drab mixes…
Q: What is the total magnification of a specimen viewed with a 10x ocular lens and a 45x objective…
A: The correct ans is 450X. The total magnification of specimen viewed with a 10x ocular lens and 45x…
Q: Explain the principle of the isoelectric focusing electrophoresis method and what it is used for.
A: Proteins are defined as the biological molecule that are produced by the building blocks amino…
Q: If the ocular lens of a microscope has 10X magnifying power, and the 40X objective lens is being…
A: Microscope is an instrument used to visualize minute objects . It consists of two types of lens…
Q: What is the total magnification possible with a 10x objective?
A: The microscope is used to view the objects that are not seen through the naked eye. Microscopes use…
Q: How can one obtain 2,000× magnification with a 100× objective?
A: Magnification means how many times the image is larger than the object.
Q: What is the main application of Atomic absorption spectrophotometer (AAS)?
A: The Atomic Absorption Spectrophotometer (AAS) is primarily used to quantify the presence of several…
Q: What is the significance of putting scale bar in photomicrographs?
A: Scale bar:A scale bar is an instrument or software that attaches to a slide or image when it is…
Q: What is an electron micrograph? Why do electron micrographshave greater resolution than light…
A: Microscope helps to observe the organisms or objects that cannot be seen by our naked eye because…
Q: True- False Acetone is the safest solvent for cleaning an objective lens.
A: The micro-organism is the organism that is too small to be seen with the naked eye. It is viewed by…
Q: What is a crystal violet stain and its purpose?
A: Crystal violet is that the name given to the cluster of similar organic compounds that are used as…
Q: Can someone explain the concept behind a sample that lies in the UV range in a spectrophotometer?…
A: UV spectroscopy or UV–visible spectrophotometry refers to absorption spectroscopy or reflectance…
Q: If the total magnification of a slide is 400x and the ocular lenses are 10x, what is the magnifying…
A: Magnification is the process of enlarging the apparent size. This enlargement is quantified by a…
Q: What is the working principle of raman spectroscopy.
A: Raman spectroscopy helps in determining both qualitative and quantitative information of the analyte…
Q: Assume that you have an “X" solution that you do not know its concentration. But you have other X…
A: The spectrophotometric methods is used to measure the absorbance of a sample by measuring the…
Q: is the reason for using blind tube in spectrophotometric and calorimetric studie
A: A method which is used to measure how much a chemical substance absorbs light by measuring the…
Q: What is the principle behind a UV-Vis spectrophotometer, and what are its key components, and how…
A: UV-vis spectrophotometry or UV spectroscopy types of absorption spectroscopy or reflectance…
Q: What macromolecules does DAPI stain? What are its advantages and disadvantages?
A: Genetics is the branch of biology that deals with genetic material like DNA, RNA, inheritance.…
Q: If the field diameter is 2.4 mm and there are 16 cells across the field of view when using the 40X…
A: Given, Field diameter - 2.4 mm Cells across the field of view - 16 cells Objective Lens - 40X The…
Q: Using a good compound light microscope with a resolving power of 0.3 µm, 10X ocular lens, and a 100X…
A: Using a good compound light microscope with a resolving power of 0.3 µm, 10X ocular lens, and a 100X…
Q: For each stain, indicate if it’s a cationic dye or an anionic dye (2 pts). Stain…
A: Cationic Dyes- These are the dyes which dissociates into cations in aqueous solutions. Anionic Dyes-…
Q: What is the stationary and mobile phase of spectrophotometry?
A: Introduction A biotechnique based on the principle of quantitative measurement of light intensity…
Q: Discuss the types of interferences in atomic absorption spectrophotometry.
A: Discuss the types of interferences in atomic absorption spectrophotometry. Introduction: Spectral…
Q: What is the purpose of setting the spectrophotometer to zero absorbance using a blank solution?
A: Spectroscopy is a procedure utilized for the evaluation, portrayal, and basic investigation of a…
Q: How do photopigments separate when performing paper chromatography? Most soluble pigment will stay…
A: Paper chromatography is a technique which is used to separate the mixture by using a moving solvent…
Trending now
This is a popular solution!
Step by step
Solved in 2 steps
- What is the driving force behind paper chromatography?What is the basic purpose of the paper chromatography?Define each of the following terms: A) What is resolution and how is resolution related to the wavelength of light used to illuminate the sample? B) What is the magnification of the specimen if you are using a 40x objective and a 10x eyepiece? C) How is the numerical aperture (NA) of a lens related to its ability to gather light from a specimen?
- The following image is a scheme for serial dilutions prepared for spectrophotometric analysis. If the stock solution concentration is 0.05 % (v/v) can you calculate the other tube’s concentrations in % v/v? I've used this with direct dilutions, how would I use this on serial dilutions?What is thin layer chromatography?NMR is considered a low-sensitivity spectroscopic technique. What is the physical basis of NMR's insensitivity?
- What is the difference between normal and reverse phase chromatography?Describe the key components and principles of operation of a x-ray unit used for film/screen mammography.Use diagrams,where appropriate to illustrate your answerWhat is the difference between Raman Spectroscopy and SERS ( Surface Enhanced Raman Spectroscopy).
