It may seem contradictory that lyophilization can be used to preservefoods yet also be used to keep cultures of microbes viable. Explainhow these applications can be reconciled.
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It may seem contradictory that lyophilization can be used to preserve
foods yet also be used to keep cultures of microbes viable. Explain
how these applications can be reconciled.
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Solved in 2 steps
- After inoculating and incubating an agar slant from a pure broth culture of a bacterial species such as E. coli, which of the following would indicate an unsuccessful aseptic transfer? (Choose ALL that apply) a - There is fungal growth in the original broth culture tube. b- There is too much growth on the agar slant. c- There are colonies of similar morphology on the slant. d - There are red, yellow, and white colonies on the slant. e - There is no growth on the slant.The thermal death point is best described as:(a) The highest temperature that will support growth of amicrobial culture(b) The length of time required for killing 100% of mi-crobes in a 24-hour culture that is heated to 100°C(c) The temperature at which a previously growing 24-hourculture will begin to die(d) The temperature at which 100% of the microbes in a24-hour culture will be killed in 10 minutesBased on the knowledge acquired about the conservation and propagation of microorganisms, propose methodologies for:a) conservation of stock culture (used infrequently) of baker's yeast (S. cerevisiae);b) conservation of the working culture (used daily) of the baker's yeast;c) activation (1st stage of propagation) of the work culture;d) propagation of cultures until inoculating a 100 L reactor toi) production of baker's yeast;ii) ethanol production.
- (1) What is the difference between specific growth rate and generation time? (2) Why is there a need to adjust the optical density of a pre-culture against a McFarland standard or measure it using a spectrophotometer?Is the Tryptoc Soy Agar (TSA) a complex defined medium? Explaining based on its composition? Is TSA a differential, selective, both differential and selective media? Please help by explaining based on what kind of MicroOrganisms it allows to grow?There are media that can be both differential and selective. what are 3 examples and the function of each one of them?
- distinguish between selective, differential, enriched, and minimal media. Give examples of Distinguish between selective, differential, enriched, and minimal. Give examples of each. know on what the selective and differential properties are based.A soil sample is placed in liquid and the number of bacteria in the sample determined in two ways: (1) colony count and (2) direct microscopic count. How would the results compare?a) Methods 1 and 2 would give approximately the same results.b) Many more bacteria would be estimated by method 1.c) Many more bacteria would be estimated by method 2.d) Depending on the soil sample, sometimes method 1 would be higher and sometimes method 2 would be higher.Can simply observing the colonies formed on agarplates and the results of a few biochemical testsconfirm how many different types of bacteria arepresent in coconut water samples? Explain.
- Briefly outline the mechanism leading to the occurrence of bioluminescence by these microbes.Is the phenylethylalcohol agar (PEA) a complex or defined medium? Please help explain based on what kind of microorganisms it allows to grow.Is generation time a useful parameter to indicate the types of mediabest suited to support the growth of a specific organism? Explain.