Prior to the publication of the article from which this assay comes from (picture), the Cas9 endonuclease was known to be involved in CRISP RNA (crRNA) maturation, but the mechanism by which Cas9 participates in targeted DNA disruption was unknown. To test whether and how Cas9 might be able to target DNA cleavage, researchers developed an in vitro assay. One of the first steps was to purify the Cas9 protein from Streptococcus pyogenes and several orthologs. The description of the figure states: SDS-PAGE analysis of purified Cas9 orthologs. Cas9 orthologs were purified as described in Supplementary Materials and Methods. 2.5 ug of each purified Cas9 were analyzed on a 4-20% gradient polyacrylamide gel and stained with SimplyBlue Safe Stain. Answer the following questions based on this information and the figure. a. Results: Explain the data in terms of observations and patterns. b. Conclusion: Describe how the data support or not support the hypothesis. c. Briefly describe a next experiment to be performed based on the conclusion

Prior to the publication of the article from which this assay comes from (picture), the Cas9 endonuclease was known to be involved in CRISP RNA (crRNA) maturation, but the mechanism by which Cas9 participates in targeted DNA disruption was unknown. To test whether and how Cas9 might be able to target DNA cleavage, researchers developed an in vitro assay. One of the first steps was to purify the Cas9 protein from Streptococcus pyogenes and several orthologs. The description of the figure states: SDS-PAGE analysis of purified Cas9 orthologs. Cas9 orthologs were purified as described in Supplementary Materials and Methods. 2.5 ug of each purified Cas9 were analyzed on a 4-20% gradient polyacrylamide gel and stained with SimplyBlue Safe Stain. Answer the following questions based on this information and the figure. a. Results: Explain the data in terms of observations and patterns. b. Conclusion: Describe how the data support or not support the hypothesis. c. Briefly describe a next experiment to be performed based on the conclusion