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- A mixed culture is known to contain Gram negative bacilli and Gram positive cocci. You perform a Gram stain of this mixed culture as a control while simultaneously Gram stainin an unknown organism on the same slide (the same procedure you used in lab). The results, observed under the 100x objective lens, are described below: Results: Control side: purple bacilli and purple cocci Unknown side: purple bacilli Using all the information given above, discuss the results of your unknown organism. Shoul you be confident in this result? Answer question A if you are confident, answer question B if you are not confident. a. If you are confident in your results, why? Explain how the staining reagents produced the described results. b. If you are not confident, do you think an error was made in the Gram staining procedure? If so, describe the error and explain how it impacted your results.How can colony morphology be used in the identification of bacterial species? What are the environmental factors that affect the growth of bacteria? What is agar (or agar-agar)? Is this different from nutrient agar? Why? How is xylol used to clean the lens of a microscope? What may happen if xylol is constantly used for this purpose? For what purpose would you adjust each of the following microscope components during a microscopy exercise? iris diaphragm coarse adjustment knob fine adjustment knob condenser mechanical stage controlYou have identified two colony types A and B on the streak plate. Now briefly describe (in 3-4 sentences) the process of how you identified the cellular morphology/arrangement of each isolate. Again, assume you have all the necessary equipment and materials at your disposal. Be concise and thorough, not verbose; e.g., refer to the Gram stain, but describing the details of each step is not necessary.
- When performing a Gram stain on a Gram-negative bacteria, why does the bacteria stain pink? Crystal violet stain binds to iodine and remains in the bacterial cell wall because of the thick peptidoglycan layer. Safranin counterstain gets in the bacteria’s cell wall to stain the cell pink. The alcohol wash removes crystal violet and iodine from the bacteria. Crystal violet stain binds to iodine and remains in the bacteria cell wall because of the thick peptidoglycan layer AND Safranin counterstain gets in the bacteria’s cell wall to stain the cell pink. Safranin counterstain gets in the bacteria’s cell wall to stain the cell pink AND The alcohol wash removes crystal violet and iodine from the bacteria.Why is Gram staining so important for bacterial characterization (i.e. what does it tell us)? In one paragraph, describe the difference between Gram positive, Gram negative, and capsule surface structures.What is the process to perform a Gram Stain? What happens if you make a mistake on a step (Think about different mistakes you could make)? What are the proper results? What structures do Archaeans use to attach to surfaces (Look like treble hooks)? What are bacterial inclusions? What are the similarities and differences in Gram - cells and Gram + cell structure? What is the function of ribosomes and what are they mad up of? What component of bacterial cells helps to combat/regulate osmotic forces? What are Koch’s postulates? What are they used for? Explain the process of endospore formation in endospore-producing organisms. What are the major components of the bacterial & eukaryotic cell? What is/are a pilus/pili and what do microbes use them for? What is the prokaryotic flagellum made up of?
- After conducting the Gram staining procedures and you observe nothing (you didn’t see anything) in your slide under the microscope, what could be the mistake you have done? What can you observe when you view a Gram stained-mixture of bacterial specimen?You have a culture of bacteria and you performed an Acid Fast staining. You see red rod-shaped bacteria afterwards. You can infer that: It is an Acid-Fast negative bacteria and it is Gram negative It is Acid-Fast negative and it is Gram Neg ive It is an Acid-Fast positive bacteria and it is Gram positve It is Acid-Fast positive and it is Gram negative It is an Acid Fast positive bacteria but its Gram stain property is unknownFor the picture below, answer the following questions: A. What is the shape of the bacteria? B. What is the arrangement of the bacteria? C. If this slide shows a Gram stain, what is the Gram stain reaction? If this slide does not show a Gram stain, please state that as your answer. ASM MicrobeLibrary.org Smith
- What is the difference between gram positive and gram negative bacteria? Describe how we use this to our advantage in the gram staining process. Hint: Gram positive versus Gram negative!!!You receive a bacterial culture in the lab and your job is to figure out information which will be helpful. You are only allowed to use one of the staining methods we practiced. Which one would be the best option? Why? And what will this information help you determine? Explain.How would you expect the staining properties of 24-hour culture of Bacillus subtilis or the other Gram-positive bacteria to compare to culture that is 3 to 4 days older?