The statement, “In the laboratory, a sterile inoculating loop is moved across the agar surface in a culture dish, thinning a sample and isolating individuals", describes which technique?
Q: Why is it important to limit the quantity of cells used to prepare a smear?
A: A thin layer of cells or tissues that are taken from the body of an organism under study which is…
Q: You aseptically transfer 1 mL of your original liquid culture into 99 mL of sterile water. What is…
A: The dilution factor is a term used to describe the ratio of the final volume over the aliquot…
Q: What is a mixed culture? A contaminated culture?
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Q: What is the purpose of the pour plate technique? If a pure culture is used to inoculate the plate,…
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Q: Why is dilution a necessary part of pure culture preparation?
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Q: What is the amount (mL) of pre-culture (108 cell/mL) necessary to inoculate (to add) in a 2-liter…
A: Culture media is a solid or semisolid substance used to support the growth of microorganisms. It is…
Q: Why does a streaking method used to inoculate plates result in isolated colonies?
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Q: What is the difference between culture-based technique and culture-independent techniques based on…
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Q: FNA
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Q: What order should the steps be in for this culture method?
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Q: In this experiment, a culture was serially diluted to the concentrations below. Each plate was…
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Q: How can the enrichment culture technique be used medically?
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Q: How will you prevent the occurrence of the bacteria present in the Rapid Strep A Test?
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Q: procedure/s in performing aseptic transfer of bacterial cultures in (include illustration) (3) agar…
A: Aseptic Technique describe some of the ways that a laboratory can deal with the constant threat of…
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Q: Why do we use the term CFU (colony forming unit) instead of reporting bacteria per ml? Explain.
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Q: Explain why it is important to use only a small amount of bacteria when preparing a smear
A:
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Q: Which of the media used in these labs (MSA, BAP, CAP) was selective? differential? highly enriched?
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Q: If there is 50 bacterial colonies on a 1:1000 dilution streak plate. How many cfu/mL are there?
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Q: Please discuss how the Methyl Red-Vogues Proskauer test helps distinguish microbes from each other.
A:
Q: What is the purpose of adding sodium bicarbonate to a culture medium?
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Q: you are given a mixed culture of s. aureus, E. coli and P. aeruginosa. how would you isolate each…
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Q: in an indirect elisa procedure what enzyme is used?
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Q: how to find out the test is differential or selective in the microbiology lab test?
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Q: How does smear preparation of cells from a liquid medium differ from preparation of cells from a…
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Q: discuss why a mixed culture cannot be used to inoculate a differential media such as the tripple…
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Q: Why are plated media stored in an inverted position?
A: Plated media enables the isolation of microorganisms from samples or specimens.
Q: Name three reasons why the ELISA test is useful in detecting microbes
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A:
Q: Why did you perform the catalase test on colonies growing on nutrient agar plates but not on the…
A: Catalase test is used to identify the presence of catalase enzyme in the bacteria. Catalase is an…
Q: What is the goal of isolation streak plate technique? Why is microbial considered a pure culture?
A: Streaking : Streaking for isolation on an agar plate involves the successive dilution of organisms…
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- A bacterial culture was grown for 12 hours. At 4-hour interval, the culture was sampled to determine the population of the culture, by transferring 25 ml of the suspension to 225 ml 0.90% NaCl. Three consecutive dilutions were further made by using I ml aliquot in 9 ml of 0.90% NaCl. One ml from each dilution was plated in each of duplicate plates. The following table shows the results of the plating method. 田 Sampling COUNTS 1st dilution 54; 61 2nd dilution 3rd dilution 4h dilution 1st 3; 7 0; 0 0,0 2nd TNTC TNCT 242: 233 28: 37 3rd TNTC TNTC INTC 249-246 * TNTC = Too numerous to count i. Illustrate the dilution series used and label the final dilution of each dilution. ii. Determine the bacterial count (CFU/ml) every 4 hours of incubation for 12 hours. Show all computations.You are given a mix culture of S. aureus,E. coli and P. aeruginosa. Besides the streak plate method what other methods could you use to separate the bacteria? Please state what method(s) you would use, the result you would be looking for to help identify each bacterium and the interpretation of the result.You are given a bacterial culture which has a concentration of approximately 5.0 x 10^8 cells/mL. List a series of dilutions and platings that you could carry out in order to determine the exact concentration of the culture. Note that you must plate four plates from a minimum of two dilution tubes. The volumes plated should be in the range of 0.1 mL – 1.0 mL. Duplicate volumes may not be plated from any one dilution tube. Each plating should aim for a count between 30 and 300 CFUs. You can select any value from 30-300 for CFU and any volume from 0.1-1.0 to find out dilution scheme
- (1) why can't we say "sterile" technique (2) how are aseptic technique similar and different in the lab and Healthcare field?Be specific and explain at least 2 differences and two similarities. (3) You are asked to develop a method of transfer an unknown organism from a liquid broth to a solid petri dish.list each step that you would have to take .be specificHow do eosin-methylene blue (EMB) agar plates work? What organism(s) are they designed to detect? How would a positive test appear on the plate?Based on the found under #4 inoculating plates order, and the definitions above, what can you assume the order of plating cultures is based on? *
- during inoculation, the bacterial culture tube is always held at an angle and the lid of the Petri dish is slightly open. Explain the purpose of these steps briefly.Why go to the trouble of creating a master plate (why not simply plate the initial culture on both nutrient agar and glucose-salts agar)?You were instructed to add 1.0ml out of 4.0ml of an undiluted sample to 99ml of sterile diligent you add the entire 4.0ml to 99 ml. a) what was your intended dilution factor? b) what was your actual dilution factor?
- In the ELISA, the pH the coating buffer was 9.6 whereas the pH of the sample buffer (PBS based) was 7.4 a) Why was the pH of the coating buffer so different?b) What is “coating buffer” for an ELISA? Does the buffer molecule usedmake sense based on the desired pH?In an ELISA procedure the samples are incubated and the ELISA plate iscovered with parafilm and placed in a humidified chamber to preventevaporation of the small liquid volumes in the wells.c) How would your results change if you did not incubate in a humidifiedchamber?Refer to the provided image drawn by a student trying to plan out their serial dilution protocol. The student diluted the original culture into bottle A and then diluted it further into B as shown. The student then proceeded with plating out 0.1ml of the culture from bottle B onto the plate (Note: plate A shows the 0.1ml that was plated, no further dilutions were done). If 13 colonies grew on plate A, help the student figure out how many CFU (colony forming units) were in the original culture? Select one: a.1,300 b.13,000 c.None of the Above d.130,000 e.1,000,000Explain why culture based biochemical and genetic testing would not be the ideal method to identify the microbe.