What is the color result of 1% solution of casein, Albumin, Gelatin in ninhydrin test? Include picture and interpretation
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- A solution of flycine and a solution of albumin were prepared with the same concentration (g/L). One of the soutions turned light purple and the other dark purple for hte ninhydrin test. Which one would you expect to give the darker purple color? Explain.Please explain the theoretical background (positive color reactions and what is being detected) involved in the different qualitative test for proteins that has been isolated intact or hydrolyzed. Please directly answer the theoretical background (positive color reactions and what is being detected) of each test GENERAL: BIURET - NINHYDRIN SPECIFIC XANTHOPROTEIC TEST - MILLON'S TEST - HOPKINS-COLE TEST - SAKAGUCHI TEST - LEAD ACETATE TEST - NITROPRUSSIDE TEST - FOLLIN'S REACTION - SULLIVAN'S TEST - PAULY REACTION - LIEBERMAN'S TEST -4 mL of 10% TCA solution was added to 1 mL of serum and after mixing, it was waited for two minutes and filtered through non-phosphorus filter paper. 1 mL of the filtrate was taken and 13 mL of distilled water, 4 mL of sulfomolybdic acid and 2 mL of dilute SnCl2 solution were added and mixed, and after waiting for 15 minutes, the absorbances of the obtained solutions against pure water at 520 nm were read. If the function of the calibration graph obtained with 0.5-2.5 mg/mL standard phosphorus solutions is y= 0.245x + 0.107 and the absorbance value of the serum sample is 0.109, what is the amount of phosphorus in the sample?
- In outline form discuss the steps of Ziehl-Neelsen staining Complete the table for National Reporting Scale (Description) National Reporting Scale Result Description 0 +n 1+ 2+ 3+Provide the Experimental procedure of the following: Example: Ninhydrin Test -The samples needed in this test were 1% casein solution, albumin and gelatin and the reagent used was ninhydrin (Triketohydrindenehydrate or Triketohydrin hydrate). Test tube, test tube stand, pippete, water bath, and spectrophotometer were the materials and equipment used. Ninhydrin reagent in 1-2 drops was added to 0.5 ml of the sample with care. It was mixed and heated to boiling for 1-2 minutes. Then, it was allowed to cool to record the observation. Test that needs Experimental procedure: 1. Heat denaturation Samples: Albumin, gelatin 2. Isoelectric Precipitation Samples: Casein 3. Concentrated Mineral Acids Samples: Albumin 4. Organic Solvents Samples: AlbuminWhat does translucent spot mean with regards to lipid? Identify the control for this test. 1. Oil 2. Cheese 3. Water 4. Cheese
- Differentiate Wintrobe from Westergren regarding accuracy of the test in a tabulated order.Give the procedure of Sabin-Feldman dye test. Describe the positive and negative results.What is the purpose of Xanthoproteic test and explain the observation or rection for albumin and gelatin in terms of principle of xanthoproteic test.