What is the composition of minimal initiation complex during transcription? TBP, TAF, TFIIA, TFIIB, TFIIF O TFIIA, TFIIB, TFIIF, RNA Pol II TEIID, TFIIA, TEIIB, TFIIF TEIID, TFIIA, TEIIB, TFIIF, RNA Pol II
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- Which of the following is/are true regarding transcription initiation in bacteria? TFIID will bind the promoter at the -10 and -35 consensus sequences, then it recruits RNA polymerase Il to initiate transcription. RNA polymerase core enzyme binds the promoter at the -10 and -35 consensus sequences, then initiates transcription with the help of the sigma subunit. TFIID binds the promoter at the TATA box, then recruits RNA pol Il to initiate transcription. The sigma subunit protein of the RNA polymerase holoenzyme binds the promöter at the -10 and -35 consensus sequences, then the holoenzyme unwinds the DNA and initiates RNA Svethocie Bacteria are only able to initiate transcription of genes when ribosomes are available. Ov.The following segment of DNA is part of the RNA-coding sequence of a transcription unit. If the bottom strand is template, which of the following RNA sequences would be transcribed? DNA: 5-'ATAGGCGATGCCA-3' 3'-TATCCGCTACGGT-5' O 5'-UAUCCGCUACGGU-3' O 5'-ACCGUAGCGGAUA-3' O 5'-AUAGGCGAUGCCA-3' O 5'-UGGCAUCGCCUAU-3'The sigma-70 promoter is very well-studied. The Sigma-54 promoter, on the other hand, hasn't had quite as much study devoted to it, but a consensus sequence for Sigma-54 promoters has been determined, and it looks like this: C GAAA FICGCACTECT УА C 5-26-25-24 -23 -22 -21 -20 -19 -18 -17 -16-15-14-13-12-11 -10 3 wablogo berkalay.edu (From Franke et. Al, 2011 BMC Genomics) If you were studying a gene with a promoter that exactly matched the consensus sequence, which base would, if mutated, probably make the largest difference in the ability of Sigma-54 to bind to a promoter? O Base position 26 Base position 10 Base position 12 Base position 16
- TAN TTGC IGGAG Nanog regulatory sequence Given this interaction map which DNA sequence would the transcription factor most likely bind to? CAAGGAG ATTAACG TAATTGG TAATTGCIn which strand is the promoter? 5' TCATGAGATA GCCATGCATA TCTAGTATGT AGGCATCTGA GTTTATATCT CA 3’ 3' AGTACTCTAT CGGTACGTAT AGATCTATCA TCCGTAGACT CAAATATAGA GT 5'During the process of bacterial transcription initiation, an open complex is formed involving the promoter and RNA polymerase. The open complex ________________________________. is the first complex formed between RNA polymerase and the promoter is formed after the sigma factor is released is an unstable transcription initiation complex formed between RNA polymerase and the promoter in the absence of sigma factor is a transcription complex in which an RNA stem-loop structure keeps the DNA strands separated. is a stable transcription initiation complex in which the DNA strands in the promoter region are separated
- Which of the following is a property or characteristic of eukaryotic promoters? They contain the -10 and-35 consensus sequences where RNA polymerase holoenzyme, containing the sigma factor, bind to initiate transcription. | They contain the -10 and -35 consensus sequences that are the site where general transcription factors bind to help RNA polymerase holoenzyme initiate transcription. They contain a TATA box to which sigma factors bind to help RNA polymerase core enzyme initiate transcription. O I. They are usually located far away from the gene that is being transcribed. IV. OV. A, They are bound by general transcription factors, which help RNA polymerase I, II, or III, bind to the promoter and initiate transcription.During eukaryote transcription initiation, when TFIIE, TFIIA, TFIIB, TFIIF, and RNA pol II all join on the DNA strand, how do they know where to be put on the strand?Why is an NFR needed at the core promoter for transcription to occur?
- A pre-initation complex (PIC) is required for transcription to begin. State which TFII protein has catalytic function.Below is the double stranded DNA sequence of part of a hypothetical yeast genome encoding a very small gene. Transcription starts at nucleotide immediately following the promoter. The termination sequence is TATCTC. How many amino acids will this protein have? 5' TCATGAGATA GCCATGCACTA AGGCATCTGA GTTTATATCT CA 3' 3' AGTACTCTAT CGGTACGTGAT TCCGTAGACT CAAATATAGA GT 5'How does the 4 feature of transcription factors namely the structural motifs of DNA binding protein, activation domains, multiple transcription factors and enhancers help in the design of a building block tool. U can use the SrY gene as ur building block tool. Pls explain in details using those features of the transcription factors. In 400 words