Which of the following about the splicing in posttranscriptional processing is NOT true? O a. O b. O C. In bacterial, the genes coding for polypeptides are interrupted by non-coding regions. Introns in eukaryotic hnRNA must be cut out before mature mRNA can be used for protein synthesis. Alternate splicing can lead to tissue-specific proteins.
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- Explain how each of the following processes complicates the concept of colinearity. Q. Alternative splicingThe method of Northern blotting is used to determine the amountand size of a particular RNA transcribed in a given cell type.Alternative splicing (discussed in Chapter 14) produces mRNAsof different lengths from the same gene. The Northern blot shownhere was obtained using a DNA probe that is complementary tothe mRNA encoded by a particular gene. The mRNA in lanes 1through 4 was isolated from different cell types, and equal amountsof total cellular mRNA were added to each lane. Explain these results.How is the splicing of U2-dependent introns different from that of U12-dependent introns? O a. U12-dependent introns are self-splicing. O b. U2-dependent introns have longer consensus sequences at the splice sites. O.U12-dependent introns have no branch site. d. The SR proteins in the spliceosomes are different. e. Most of the snRNPs in the spliceosomes are different.
- What is the order of the tRNA binding sites on the 70S ribosome with respect to the 5'→ 3' direction of mRNA? O a. PAE ОБ.ЕРА OC. AEP O d. PEA O e. E AP Which of the following is TRUE regarding eukaryotic RNA processing? O a. Functional individual FRNA cannot be produced without splicing. O b. Splicing of the MRNA precursor is done in the cytoplasm, while modification of the ends is done in the nucleus. OC. An unprocessed mRNA precursor is translocated into the cytoplasm to be modified before the translation. O d. Splicing of the mRNA precursor is done in the nucleus, while modification of the ends is done in the cytoplasm. O e. Transcription yields primary transcript, which can by itself yield a functional protein. RNA self-splicing demonstrates the role of RNA as a(n): O a. spliceosome. O b. intron. O c. nucleophile. O d. exon. O e. catalyst.Which of the following statements are true about eukaryotic mRNA?a. The sigma factor is essential for the correct initiationof transcription.b. Processing of the nascent mRNA may begin beforeits transcription is complete.c. Processing takes place in the cytoplasm.d. Termination is accomplished by the use of a hairpinloop or the use of the rho factor.e. Many RNAs can be transcribed simultaneously fromone DNA templateIS. Alternative splicing has been estimated to occur in more than 95% of multi-exon genes. Which of the following is not an evolutionary advantage of alternative splicing? Alternative splicing increases diversity without increasing genome size Different gene isoforms can be expressed in different tissues Alternative splicing creates shorter mRNA transcripts Different gene isoforms can be expressed during different stages of development.
- Through alternative splicing, eukaryotes (a) reinforce gene inactivation (b) prevent transcription of heterochromatin (c) produce related but different proteins in different tissues (d) amplify genes to meet the requirement of high levels of a gene product (e) bind transcription factors to enhancers to activate transcriptionWhich of the following is/are typically removed from pre-mRNA during nuclear processing in eukaryotes? (a) upstream leader sequences (b) poly-A tail (c) introns (d) exons (e) all the precedingNascent form of the mRNA A. undergoes splicing only after capping B. is also called hnRNA C. is polyadenylated after splicing D. participates in the spliceosome assembly for splicing E. joins snRNPs to form the spliceosome assembly B, C, D, E only A, B, C only A, B, C, D, E A, B, D, E only
- If an MRNA is alternatively spliced, then different introns are removed from a pre-MRNA, producing transcripts that will potentially encode proteins with different primary structures. I. the MRNA is found in a bacterial cell. II. transcription and translation of the MRNA will occur simultaneously. the alternative splicing happened in the cytoplasm. IV. Ay different exons from a pre-mRNA will be spliced together. V. the different MRNAS will all have poly-A tails. VI.How would you describe the "purpose" of alternative splicing? Alternative RNA splicing... is a common feature of prokaryotic gene expression O b. is a mechanism for increasing the rate of transcription O c. is due to the absence of particular snRNPs d. results in the production of different versions of proteins from a single gene O e. results in the production of similar proteins from different genesWhich of the following statements about pre-mRNA splicing is FALSE? a. Splicing of an intron requires consensus sequences at the splice donor, the splice acceptor and the branch point of the intron. b. The spliceosome contains proteins and RNA molecules. c. Some snRNAs base pair with splice consensus sequences on the primary transcript. d. Splicing happens before the export of the mRNA to the cytoplasm. e. Splicing happens in the cytoplasm, when spliceosomes move down the pre-mRNA in front of ribosomes.