You perform a concentration of a single component using displacement chromatography, and the following data applies: Concentration of binding sites: 4*10^(-4) M Kd for displacer: 2.0*10^(-5) M Kd for component A: 4*10^(-5) M Kd for component B: 1.5*10^(-4) M The concentration of the injected displacer is

You perform a concentration of a single component using displacement chromatography, and the following data applies: Concentration of binding sites: 410^(-4) M Kd for displacer: 2.010^(-5) M Kd for component A: 410^(-5) M Kd for component B: 1.510^(-4) M The concentration of the injected displacer is

Principles of Instrumental Analysis

7th Edition

ISBN:9781305577213

Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Chapter26: An Introduction To Chromatographic Separations

Section: Chapter Questions

Problem 26.14QAP

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1) The ratio strength of a 10mL vial of neostigmine methylsulfate injection USP is 1:1500. Calculate the amount of neostigmine methylsulfate in milligrams contained in the 10mL vial. (Round to the whole number).
percentage purity of an ibuprofen sample was determined by a reversed phase HPLC method as follows: Y Der ● A calibration curve of peak area versus concentration (ug/mL) of pure ibuprofen was constructed The ibuprofen sample for analysis was prepared by taking 10.0204 g of powder and dissolving it in/1Lbf distilled water (solution A). 1 ml of the latter solution was further diluted to IL with distilled water (solution B). Solution B was analysed by HPLC analysis Results The equation for the best-fit line of the calibration curve is: y=26820x-1323.8. ● The average peak area for the ibuprofen sample (solution B) was 266796.2 Calculate the percentage purity of the ibuprofen powder.
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. 100 ml boiled cooled and filtered water sample takes 9.6 ml of M/50 EDTA in titration. The Permanent hardness of the water sample in terms of ppm of CaCO3 equivalent is
The results in the table below were obtained from the analyses of Mg2+ content in an orange juice sample using a flame-AAS. Based on these results answer the following questions: (i) (ii) Replicate 1 2 3 4 Mg²+(ppm) 7.08 7.21 7.12 7.09 Calculate the mean, standard deviation and coefficient of variation (or %RSD) for the results obtained. If the certified value of the Mg2+ content of the apple juice sample is 7.45 ppm. What is the percentage of recovery for this analysis?
A 0.0200 gram blood sample was decomposed by a microwave digestion technique followed by dilution to 100.0 mL in a volumetric flask. Aliquots of the sample solution were treated with a lead complexing reagent and water as follows: Solution 1: 10.0 ml blood sample + 20.0 mL complexing agent + 30.0 mL H20. Solution 2: 10.0 ml blood sample + 20.0 mL complexing agent + 26.0 mL H20 + 4.00 mL of 78 ppb Pb2+ standard. The resulting solutions were analyzed by UV/Vis at 375 nm. Absorbance for solution 1 = 0.155 and for solution 2 = 0.216. Calculate the concentration of lead (ppb) in the original sample.
Researchers used gas chromatography to determine the % v/v methyl salicylate in rubbing alcohol [Van Atta, R. E.; Van Atta, R. L. J. Chem. Educ. 1980, 57, 230-231]. A set of standard additions was prepared by transferring 20.00 mL of rubbing alcohol to separate 25 - mL volumetric flasks and pipeting 0.00 mL, 0.20 mL, and 0.50 mL of methyl salicylate to the flasks. All three flasks were diluted to volume using isopropanol. Analysis of the three samples gave peak heights for methyl salicylate of 57.00 mm, 88.5 mm, and 132.5 mm, respectively. Determine the % v/v methyl salicylate in the rubbing alcohol.
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