Concepts of Genetics (12th Edition)
12th Edition
ISBN: 9780134604718
Author: William S. Klug, Michael R. Cummings, Charlotte A. Spencer, Michael A. Palladino, Darrell Killian
Publisher: PEARSON
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Textbook Question
Chapter 1, Problem 10PDQ
Outline the roles played by restriction enzymes and vectors in cloning DNA.
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Describe the important features of cloning vectors. Explain the purpose of selectable markers in cloning experiments.
Outline the roles played by restriction enzymes and vectors in cloning DNA.
Describe the various types of vectors used in DNA cloning. Explain the difference between a cloning vector and an expression vector.
Chapter 1 Solutions
Concepts of Genetics (12th Edition)
Ch. 1 - Describe Mendels conclusions about how traits are...Ch. 1 - Review the Chapter Concepts list on p. 1. Most of...Ch. 1 - What is the chromosome theory of inheritance, and...Ch. 1 - Define genotype and phenotype. Describe how they...Ch. 1 - Given the state of knowledge at the time of the...Ch. 1 - Contrast chromosomes and genes.Ch. 1 - How is genetic information encoded in a DNA...Ch. 1 - Describe the central dogma of molecular genetics...Ch. 1 - How many different proteins, each with a unique...Ch. 1 - Outline the roles played by restriction enzymes...
Ch. 1 - What are some of the impacts of biotechnology on...Ch. 1 - Summarize the arguments for and against patenting...Ch. 1 - We all carry about 20,000 genes in our genome. So...Ch. 1 - How has the use of model organisms advanced our...Ch. 1 - If you knew that a devastating late-onset...Ch. 1 - Why do you think discoveries in genetics have been...Ch. 1 - The Age of Genetics was created by remarkable...
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Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- Outline the procedure for cloning a gene into a vector.arrow_forwardb) Describe how DNA is digested by different restriction enzymes c) Describe how gel electrophoresis is used to estimate the size of DNA fragments.arrow_forwardExplain the role of restriction endonucleases in the process of genetic engineering.arrow_forward
- List all steps involved in a cloning workflow. Name and link the appropriate enzymes to the steps at which they are used.arrow_forwardDescribe in vitro mutagenesis and its potential uses.arrow_forwardWith the use of well-illustrated diagrams, reconstruct the entire cloning process by explaining different stages of the cloning process that involves the following:a. Isolation of target DNA fragments (often referred to as inserts)b. Ligation of inserts into the plasmid, creating recombinant molecules c. Transformation of recombinant plasmids into bacteria or other suitable host for propagationd. Screening/selection of hosts containing the intended recombinant plasmid. For this stage(d), discuss the importance of a second marker that can be used for screening of genomic DNA for colonies containing the pka-1 under the principle of insertional inactivation. This should be properly explained using all the attributes of the plasmid described above.arrow_forward
- Explain why a cloning vector usually includes a selectable marker.arrow_forwardDescribe the process of positional cloning and how itallows mapping of disease-causing mutations.arrow_forwardBriefly present experimental and practical benefits of using PCR in DNA cloning process. Give some examples of clinical applications.arrow_forward
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