Laboratory Experiments in Microbiology (12th Edition) (What's New in Microbiology)
12th Edition
ISBN: 9780134605203
Author: Ted R. Johnson, Christine L. Case
Publisher: PEARSON
expand_more
expand_more
format_list_bulleted
Concept explainers
Question
Chapter 1, Problem 1CA
Summary Introduction
To analyze:
The type of microbe that is most likely to be observed when observing a sample from a lung biopsy at 1000x.
Introduction:
Lung biopsy is a medical procedure of removing a sample of cells or tissues from an affected region of the lung and observing it under a microscope for any microbe or lung cancer. The sample is initially examined under a light microscope that can magnify the sample up to 1500x while using light as the source of illumination.
Expert Solution & Answer
Want to see the full answer?
Check out a sample textbook solutionStudents have asked these similar questions
You have learned many different techniques so far this semester that allow us to identify pathogenic microbes in the clinical setting. Why do you think rapid methods are so helpful? Why are traditional tests such as metabolic tests not always used to ID clinically important microbes?
Below is an MSA plate inoculated with bacteria and grown at 37*C for 2 days. Be
pecific and thorough in your answers for parts a and b below.
The color of the plate is yellow because
b) The presence of bacterial colonies indicates that the bacteria can survive
General Instructions:Choose 1 bacteria/diseases and fill out the details. An example of an answered template is also provided for your reference.
Causative Agent and Disease Profile for S. aureus
Template and Example
ITEM
MSM
PROFILE
MICROBIAL PROFILE
I
MICROORGANISM/CAUSATIVE AGENT
Staphylococcus aureus
A
GRAM REACTION
(+)
B
OXYGEN REQUIREMENT
Facultative Aerobes
C
SIZE
1.5 µm
D
SHAPE
Cocci in clusters
E
HABITAT
Normal flora of skin/anterior nares/pharynx
F
DISCOVERY
G
MICROSCOPIC IMAGE
II
DISEASE PROFILE
Scalded skin syndrome
A
DISEASE/S
Skin and Wound Infections
Scalded Skin Syndrome
Toxic Shock Syndrome
Food Poisoning Pneumonia
B
SYMPTOMS OF THE DISEASE
A high fever · Nausea and vomiting · A rash on your palms and soles that resembles a sunburn
C
INCUBATION PERIOD
2 and 4 hours (range 30 minutes to 8 hours)
D
MODE OF…
Chapter 1 Solutions
Laboratory Experiments in Microbiology (12th Edition) (What's New in Microbiology)
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- Plastic pipet tips for micropipettes are commonly supplied in packs of 100’s. Once the pack has been opened, contamination is assumed. How can sterility of the pipet tips be ensured prior to use in microbiological procedures? METHOD:RATIONALE:arrow_forwardThere are so many microbes in a single mL of culture, it is very difficult to perform one dilution to produce countable cells. Microbiologists need to perform a dilution series, where multiple dilutions are performed in sequence to arrive at the correct dilution. Dilutions are cumulative. Multiple the series of dilutions together to find the final dilution value. If 3 serial dilutions are performed, each with a value of 0.01, what is the cumulative dilution? Express your answer as an exponent, e.g. 0.1 would be 1e-1 and 0.01 would be 1e-2arrow_forwardA batch of turkey rolls (10 lb—approximately 4.5 Kg—each) were cooked to 165°F internal temperature in bags, opened, sliced, vacuum-packaged, and stored at 40°F. The product was expected to have a refrigerated shelf life of 50 days. However, after 40 days, the packages contained gas and approximately 107 bacterial cells/g of meat. The bacterial species involved in the spoilage was found to be Leuconostoc carnosum, which is killed at 165°F. What could be the sources of the bacterial species in this cooked product?arrow_forward
- How would you identify this unknown bacteria using a flowchart and the bacteria below as a possible unknown? Bacillus cereus, Micrococcus luteus, Staphylococcus aureus, Staphylococcus epidermidis and Lactococcus lactis. Create flow chart (dichotomues) using at least 3 other biochemical tests from the following list: Mannitol salt agar, Blood agar, Starch agar, Tributyrin Agar, Gelatin, Casein agar, Indole Production, MR-VP, Citrate, Hydrogen Sulfide test, Urease test, Nitrate reduction test, Catalase test, and Oxidase test.arrow_forwardJoseph, being a know-it-all, told Mary that her method would not include all microbes. To demonstrate this, he took another 10g sample of Mary's garden and added starch. After the 1-week incubation time, he performed the same serial dilution scheme as Mary but used pour plating instead of spread plating. Shown below are the counts that he obtained after the incubation period. Compute for the CFU/g given the data below. Be sure to show complete solutions and box your final answer. Final answers should be expressed in 3 significant figures. Tube 1 135, 129 Tube 2 9,7arrow_forwardJoseph, being a know-it-all, told Mary that her method would not include all microbes. To demonstrate this, he took another 10g sample of Mary's garden and added starch. After the 1- week incubation time, he performed the same serial dilution scheme as Mary but used pour plating instead of spread plating. Shown below are the counts that he obtained after the incubation period. Compute for the CFU/g given the data below. Be sure to show complete solutions and box your final answer. Final answers should be expressed in 3 significant figures. Tube 1 135, 129 Tube 2 9.7arrow_forward
- After a series of experiments, bacterial cultures were identified for disposal. How can these be properly decontaminated prior to disposal according to biosafety guidelines? METHOD:RATIONALE:arrow_forwardHow many different types of colonies can you find on the blood agar plate? describe each. (As shown in pictures below) What possible types of bacteria ? (As shown in pictures below)arrow_forwardGreetings :) Based on the results of using Hanging-drop method in Figure 2, what microorganisms can you find? Kindly state or identify all microbes you can see, and briefly described each of them. Thank you so much!arrow_forward
- Mannitol salt agar is often used to distinguish between different species of Staphylococcus, a gram positive bacterium that is well adapted to living on dry, salty skin. Disease-causing strains of Staphylococcus ferment mannitol; non-pathogenic strains cannot use mannitol. Is the medium Defined or Complex?arrow_forwarda. What oxygen requirements do your think this bacterium will have (obligate aerobe, obligate anaerobe, facultative anaerobe, aerotolerant anaerobe, or microaerophile), and why? b. You run a thioglycollate test of your isolated bacterium. Based on your response to Part A, which thioglycollate tube best represents the result you would expect for your bacterium? Explain your response. If the result you would expect is not represented, explain what the tube should look like. c. What would you expect to be the result of a catalase test of your bacterium? d. What techniques or specialized equipment, if any, would you need to grow cultures of this bacterium?arrow_forwardWhen bacteria from a throat swab are streaked on blood agar, why is the agar stabbed several times with the loop?arrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
- Comprehensive Medical Assisting: Administrative a...NursingISBN:9781305964792Author:Wilburta Q. Lindh, Carol D. Tamparo, Barbara M. Dahl, Julie Morris, Cindy CorreaPublisher:Cengage Learning
Comprehensive Medical Assisting: Administrative a...
Nursing
ISBN:9781305964792
Author:Wilburta Q. Lindh, Carol D. Tamparo, Barbara M. Dahl, Julie Morris, Cindy Correa
Publisher:Cengage Learning