Biochemistry
9th Edition
ISBN: 9781319114671
Author: Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher: W. H. Freeman
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Chapter 1, Problem 31P
Interpretation Introduction
Interpretation:
The energy of the ionic interaction between two phosphate groups at the given values of dielectric constant should be calculated.
Concept introduction:
Under the effect of electric field, the induced polarization gets involved in the volume of the given material. The measurement of the potential energy in the form of induced polarization is termed as dielectric constant. Further, dielectric constant is calculated as the ratio of dielectric permittivity of material to that of the vacuum.
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Students have asked these similar questions
Suppose you have two genetic variants of a large protein that differ
only in that one contains a histidine (side chain pK, = 6.4) when the
other has a valine (uncharged side chain).
(a) Which would be better for separation: gel electrophoresis or iso-
electric focusing? Why?
(b) What pH would you choose for the separation?
Abosrbance (A 280)
You are interested in isolating an enzyme Ribonuclease. Inside cells ribonuclease self-associates and can
exist as dimers or trimers.
The active form of the enzyme is monomeric with molecular weight of ~ 14Kda.
In order to isolate ribonuclease, you use size exclusion chromatography column and obtain three peaks.
Indicate which peak (starting from left to right) corresponds to Monomer (M), Dimer (D) and Trimer (T)
forms of ribonuclease.
PEAK I:
PEAK II:
PEAK III:
Time
M
м
Fractions
Previous Assignment:
The figure below shows the bonding of the cytosine and guanine molecules. The O - H and H - N distances are each 0.110 nm. In this case, assume that the bonding is due only to the forces along the O - H - O, N - H - N and O - H - N combinations, and assume also that these three combinations are parallel to each other. Calculate the net force that cytosine exerts on guanine due to the preceding three combinations. Is this force attractive or repulsive?
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Electric Potential Energy: Base Pairing in DNA, IICalculate the electric potential energy of the guanine–cytosine bond, using the same combinations of molecules (O - H - O, N - H - N and O - H - N) as in the previous assignment (pls refer above)
Chapter 1 Solutions
Biochemistry
Ch. 1 - Prob. 1PCh. 1 - Prob. 2PCh. 1 - Prob. 3PCh. 1 - Prob. 4PCh. 1 - Prob. 5PCh. 1 - Prob. 6PCh. 1 - Prob. 7PCh. 1 - Prob. 8PCh. 1 - Prob. 9PCh. 1 - Prob. 10P
Ch. 1 - Prob. 11PCh. 1 - Prob. 12PCh. 1 - Prob. 13PCh. 1 - Prob. 14PCh. 1 - Prob. 15PCh. 1 - Prob. 16PCh. 1 - Prob. 17PCh. 1 - Prob. 18PCh. 1 - Prob. 19PCh. 1 - Prob. 20PCh. 1 - Prob. 21PCh. 1 - Prob. 22PCh. 1 - Prob. 23PCh. 1 - Prob. 24PCh. 1 - Prob. 25PCh. 1 - Prob. 26PCh. 1 - Prob. 27PCh. 1 - Prob. 28PCh. 1 - Prob. 29PCh. 1 - Prob. 30PCh. 1 - Prob. 31PCh. 1 - Prob. 32PCh. 1 - Prob. 33PCh. 1 - Prob. 34PCh. 1 - Prob. 35PCh. 1 - Prob. 36P
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- Suppose you have a solution of a protein, which contains a specific Tyr residue that has an actual (measured) pKa of 8.8. The protein binds a ligand by several noncovalent interactions, one of which is a hydrogen bond in which the Tyr phenolic hydroxyl group must serve as a hydrogen bond donor. Calculate the percentage of the protein molecules in which that tyrosyl residue's phenolic hydroxyl group could serve as a hydrogen bond donor at pH 8.5arrow_forwardE Threonine 6. You have identified some intermediates in threonine synthesis: A, B, C, D and E. You grow a few of your mutants in the presence of these different intermediates to determine the order in which the gene products act. Below are your results. A (+) means growth and a (-) means no growth. Given these data, draw the best possible pathway for the synthesis of threonine. The diagram should use arrows to indicate one intermediate being changed to another intermediate. Indicate which gene produces the product responsible for the conversion by listing the mutant in that gene above the arrow. Mt1 Mt2 Mt4 Mt7arrow_forwardSuppose two phosphate groups in DNA (each with a charge of 2 1) are separated by 12 Å. What is the energy of the ionic interaction between these two phosphates assuming a dielectric constant of 80? Repeat the calculation assuming a dielectric constant of 2.arrow_forward
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