To review:
The differences between results of Hershey and Chase’s original experiment and in new experiment in which 35S (radioactive isotope of sulfur) labeled both protein and DNA, whereas 32P (radioactive isotope of phosphorous) labeled only DNA.
Introduction:
Living beings transfer characters from one generation to another and the molecule responsible for transferring these characters is called genetic material. In the first half of the 20th century, scientists wondered which molecule of cell contains the genetic material; different scientists performed different experiments to find it. Hershey and Chase’s experiment gave evidence that DNA is the genetic material, after the experiments of Griffith (a substance transformed the bacteria pneumonia genetically), and the experiments of Avery and his coworkers (DNA is the genetic material that transformed nonvirulent bacteria to virulent bacteria).
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Chapter 14 Solutions
Biology: The Dynamic Science (MindTap Course List)
- Provide a brief summary of the Sanger sequencing method.arrow_forwardWhat did the Watson–Crick model suggest about the replication of DNA?arrow_forwardWith regard to Chargaff’s experiment described in Figure shown,answer the following:A. What is the purpose of paper chromatography?B. Explain why it is necessary to remove the bases in order todetermine the base composition of DNA.C. Would Chargaff’s experiments have been convincing if theyhad been done on DNA from only one species? Discuss.arrow_forward
- Consider the experiment conducted by Meselson and Stahl in which they used 14N and 15N in cultures of E. coli and equilibrium density gradient centrifugation. Draw pictures to represent the bands produced by bacterial DNA in the centrifuge tube before the switch to medium containing 14N and after one, two, and three rounds of replication in that medium. Use separate sets of drawings to show the bands that would appear if replication were (a) semiconservative; (b) conservative; (c) dispersive.arrow_forwardAccording to the Watson- Crick model how many polynucleotide chains does a DNA molecule have?arrow_forwardThe DNA chromosome in E. coli contains approximately 4 million base pairs. The average gene contains about 1500 base pairs. Use this information to calculate the following (show all work ): a) The length in meters of this chromosome. b) The approximate number of genes in the chromosome (assuming no wasted DNA).arrow_forward
- When proteins are separated using native gel electrophoresis, size, shape, and charge control their rate of migration on the gel. Why does DNA separate based on size, and why do we not worry much about shape or charge?arrow_forwardState the properties of the Watson-Crick model of DNA in the following categories: a) number of polynucleotide chains b) polarity (strand direction running same or opposite c) bases on interior or exterior of molecule d) sugar/phosphate on interior or exterior of molecule e) which bases pair with which f) right- or left-handed helixarrow_forwardUsing the first and second base key below, predict the DNA sequence given by the SOLID color sequence. For the key G = green, R = red, Y = yellow, and B = blue. Note that the first base of the sequence is already given ("A"). Give the remaining 8 bases for this sequence. A First base A CCT Second base A CGT BGY R GBRY RBG R Y (G) B Y G)(R) GB )( R )( Y ) ( G) Barrow_forward
- The given figure shows the denaturation curves for three DNA samples. A) What is the ?m of the most GC-rich DNA sample? B) Which point represents double-stranded DNA?arrow_forwardWhat were the two critical questions about genetic material in cells that Watson and Crick answered with the double heliz and how did the double heliz structure answer each question?arrow_forwardExplain the principles behind DNA isolation using silica membrane spin column from Qiagen. 2 After DNA isolation using Qiagen extraction kit, the final flow through was subjected to spectrophotometric measurements. Explain the situation if the absorbances were found to be: i) The absorbance at 260 nm is higher than absorbance at 280 nm? ii) The absorbance at 230 is higher than absorbance at 260 nm?arrow_forward
- Biology: The Dynamic Science (MindTap Course List)BiologyISBN:9781305389892Author:Peter J. Russell, Paul E. Hertz, Beverly McMillanPublisher:Cengage LearningBiology 2eBiologyISBN:9781947172517Author:Matthew Douglas, Jung Choi, Mary Ann ClarkPublisher:OpenStax