Concept explainers
Which of the following is not a property of DNA polymerase?
a. It adds dNTPs only in the 5' → 3' direction.
b. It requires a primer to work.
c. It is associated with a sliding clamp only on the leading strand.
d. Its exonuclease activity is involved in proofreading.
Introduction:
DNA (deoxyribonucleic acid) polymerase is the enzyme responsible for the replication of DNA. The enzymes incorporate the deoxyribonucleotides (dNTPs) into the newly synthesizing DNA molecule from the template DNA. The sliding clamp acts as a processivity promoter factor in the DNA replication. It is crucial for DNA polymerase binding to the template DNA as it prevents the frequent dissociation of the DNA polymerase from the template DNA.
Answer to Problem 1TYK
Correct answer:
The association with the sliding clamp only on the leading strand is not the property of DNA polymerase.
Explanation of Solution
Explanation/Justification for the correct answer:
Option (c) is given as association of DNA polymerase only with sliding clamp on the leading strand. The DNA polymerase is associated with the sliding clamp protein in the leading as well as with the lagging strand. Hence, the Option (c) is correct.
Explanation for incorrect answers:
Option (a) is given as adding of dNTPs only in the
Option (b) is given as primer is needed for DNA polymerase. The primer is an oligonucleotide (short stretch of DNA or RNA [ribonucleic acid]) and is required for the synthesis of DNA using DNA polymerase. So, it is an incorrect option.
Option (d) is given as proofreading is associated with exonuclease activity of DNA polymerase. The enzyme also has an exonuclease activity for proofreading of newly synthesized DNA. These are the property of DNA polymerase. So, it is an incorrect option.
Hence, the options (a), (b), and (d) are incorrect.
DNA polymerase catalyzes the addition of dNTPs in the
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Chapter 15 Solutions
Biological Science (7th Edition)
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- One common feature of all DNA polymerases is that they a. synthesize DNA in the 3′-to-5′ direction. b. synthesize DNA in the 5′-to-3′ direction. c. synthesize DNA in both directions by switching strands. d. do not require a primer.arrow_forwardA cloned fragment of DNA was sequenced by using thedideoxy chain-termination method. A part of the autoradiogram of the sequencing gel is represented here.ddA ddG ddT ddCa. Deduce the nucleotide sequence of the DNAnucleotide chain synthesized from the primer. Label the5′ and 3′ ends.b. Deduce the nucleotide sequence of the DNAnucleotide chain used as the template strand. Label the5′ and 3′ ends.c. Write out the nucleotide sequence of the DNA doublehelix (label the 5′ and 3′ ends)arrow_forwardThe short Okazaki fragments are Select one: a. spliced together by DNA ligase b. glued together by RNA primers c. fused together by DNA polymerase d. formed into the lagging strand without splicingarrow_forward
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