Life: The Science of Biology
11th Edition
ISBN: 9781319010164
Author: David E. Sadava, David M. Hillis, H. Craig Heller, Sally D. Hacker
Publisher: W. H. Freeman
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Chapter 18.2, Problem 1R
Summary Introduction
To review:
The comparison of green fluorescent protein (GFP) as a reporter gene and antibiotic resistance gene with respect to detection of recombinant deoxyribonucleic acid (DNA) in host cells.
Introduction:
In recombinant technology, to know, which host cells contain recombinant DNA, a method of detection is needed. The problem is solved by reporter genes. Their expression is easy to visualize and hence they help to detect the host cells with recombinant DNA. The most common of these reporter genes is GFP. Another most common method is the use of selectable markers like antibiotic resistance gene.
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