Biochemistry: Concepts and Connections (2nd Edition)
2nd Edition
ISBN: 9780134641621
Author: Dean R. Appling, Spencer J. Anthony-Cahill, Christopher K. Mathews
Publisher: PEARSON
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Chapter 2, Problem 20P
A biochemical reaction takes place in a 1.00 ml solution of 0.0250 M phosphate buffer initially at pH = 7.20 (see Table 2.6 for pkas of phosphate species).
a. Are the concentrations of any of the four possible phosphate species negligible? If so, identify them and explain your answer.
b. During the reaction, 3.80 µmol of HCI are produced. Calculate the final pH of the reaction solution. Assume that the HCI is completely neutralized by the buffer.
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A monoprotic weak acid, HA, dissociates in water according to the reaction
HA(aq) = H*(aq) + A¯(aq)
The equilibrium concentrations of the reactants and products are [HA] = 0.290 M, H+| = 3.00 × 10¬4 M, and
[A-] = 3.00 x 10-4 M. Calculate the value of pKa for the acid HA.
pKa
Identify the acid and conjugate base in each reaction. Calculate the pKa
for each acid. List them in order from the strongest to weakest acid. The
acid-ionization constants, Ka, at 25°C are listed for each.
a. HC2H3O2 + H2O ↔ H3O+ + C2H3O2-acetic acid, KA = 1.7 x 10-5
b. HC7H5O2 + H2O H₂O+ + C7H5O2-benzoic acid, KA= 6.3 x 10-5
c. HC6H4NO2 + H2O ↔ H3O++ C6H4NO2-nicotinic acid, KA =
1.4 x 10-5
A monoprotic weak acid, HA, dissociates in water according to the reaction
HA(aq) = H+ (aq) + A¯(aq)
The equilibrium concentrations of the reactants and products are [HA] = 0.220 M, [H+] = 3.00 × 10−4 M, and
[A¯] = 3.00 × 10−4 M. Calculate the value of pKa for the acid HA.
pKa
=
Chapter 2 Solutions
Biochemistry: Concepts and Connections (2nd Edition)
Ch. 2 - Suppose a chloride ion and a sodium ion are...Ch. 2 - Draw two different possible hydrogen-bonding...Ch. 2 - Prob. 3PCh. 2 - 4. What is the pH of each of the following...Ch. 2 - Prob. 5PCh. 2 - The weak acid HA is 2% ionized (dissociated) in a...Ch. 2 - 7. Calculate the pH values and draw the titration...Ch. 2 - What is the pH of the following buffer mixtures?...Ch. 2 - a. Suppose you wanted to make a buffer of exactly...Ch. 2 - Prob. 10P
Ch. 2 - You need to make a buffer whose pH is 7.0, and you...Ch. 2 - Describe the preparation of 2.00 L of 100 glycine...Ch. 2 - Carbon dioxide is dissolved in blood (pH 7.4) to...Ch. 2 - What is the molecular basis for the observation...Ch. 2 - The anno acid arginine ionizes according to the...Ch. 2 - It is possible to make a buffer that functions...Ch. 2 - A student is carrying out a biological preparation...Ch. 2 - Histidine is an amino acid with three titratable...Ch. 2 - Prob. 19PCh. 2 - A biochemical reaction takes place in a 1.00 ml...Ch. 2 - Is RNA-binding enzyme RNase A more likely to have...Ch. 2 - Consider a protein in which a negatively charged...Ch. 2 - Prob. 23PCh. 2 - Prob. 24P
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