Concept explainers
To review:
The functions of restriction endonucleases.
Introduction:
The nucleases are the enzymes that cut the
Explanation of Solution
Restriction endonuclease enzymes recognize a particular sequence within the genome. The specific sites recognized by these enzymes are called recognition sites. They cut the DNA at the site same as the recognition site or at any other site. The site where the DNA is cleaved is called the restriction site.
The DNA fragments produced after the cleavage by restriction enzymes are called restriction fragments. These enzymes are a part of defense mechanisms in bacteria. The DNA of viruses, which invade the bacteria, is cleaved by the restriction enzymes, and thus, the viral infection in bacteria is prevented.
The restriction endonucleases are used in the biotechnological applications.
The restriction endonucleases cleave the DNA at specific sites. The fragments produced by restriction can be used for cloning purposes to obtain recombinant DNA molecules. This enzyme also provides a defense to the bacteria against viral infection.
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Chapter 20 Solutions
Biological Science (7th Edition)
- Why do bacteria make restriction endonucleases? What is it about the endonucleases that prevents bacteria from destroying their own DNA?arrow_forwardWhat are palindromes, and how are they related to restriction endonucleases? Why are they useful for inserting one piece of DNA into another?arrow_forwardWhy do eukaryotic cells do not contain restriction enzymes?arrow_forward
- What do you mean by restriction fragments?arrow_forwardWhat is a plasmid? How are plasmids involved in bacterial transformation?arrow_forwardWhat does it mean if a restriction enzyme produces ‘sticky’ or ‘blunt’ ends? Does the restriction enzyme HaeIII produce “sticky” ends or “blunt” ends?arrow_forward
- Human Biology (MindTap Course List)BiologyISBN:9781305112100Author:Cecie Starr, Beverly McMillanPublisher:Cengage Learning