Principles of Biology
2nd Edition
ISBN: 9781259875120
Author: Robert Brooker, Eric P. Widmaier Dr., Linda Graham Dr. Ph.D., Peter Stiling Dr. Ph.D.
Publisher: McGraw-Hill Education
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Question
Chapter 4.3, Problem 1BC
Summary Introduction
To analyze:
Theeffect of alternative splicing on the structure and function of the cell.
Introduction:
The methods of controlling the part of DNA to be transcribed are referred to as gene expression. The gene expression is regulated by binding of the protein to specific sites on the DNA molecule and synthesizing regulator gene that encodes for the synthesis of the molecule known as the repressor molecule.
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19. In eukaryotic gene expression, non-coding RNAS such as miRNA (microRNA) have an
important role in gene regulation. That role is to ...
A) bind to target mRNA to prevent translation
B) bind to target mRNA to prevent transcription
C) block RNA polymerase from reaching the promoter
D) increase levels of chromatin packing
i)Describe attenuation control and how it is used to regulate gene expression.
ii)Give a specific example of how this works?
iii)Could this be used in eukaryotes? why ?or why not?
1. describe genome packaging of prokaryotes?
2. what is the role of topoisomerase?
3. what is the difference between nucleosome and chromatosome?
4. Compare the feature of euchromatin and heterochromatin?
5. Describe CPG islands?
6. In genes that are rapidly transcribed, methylation take place on what histone and what
amino acids? What is this methylation role?
7. Describe the interactions between DNA and histones?
8. describe the following words: Domain, MAR, Positive supercoil, Insulator elements
9. Compare the regeneration of nucleosomes in the transcription and replication process by
mentioning the name of the corresponding enzyme complex.
Chapter 4 Solutions
Principles of Biology
Ch. 4.1 - With regard to the origin of life, why are...Ch. 4.1 - Prob. 2CCCh. 4.1 - Prob. 3CCCh. 4.1 - Prob. 1BCCh. 4.1 - Prob. 4CCCh. 4.1 - Prob. 1TYKCh. 4.1 - Prob. 2TYKCh. 4.1 - Prob. 3TYKCh. 4.2 - Prob. 1CCCh. 4.2 - Prob. 1TYK
Ch. 4.2 - Prob. 2CCCh. 4.2 - Prob. 2TYKCh. 4.3 - Prob. 1BCCh. 4.3 - Prob. 1CCCh. 4.3 - Prob. 2BCCh. 4.3 - Prob. 1TYKCh. 4.3 - Prob. 2TYKCh. 4.3 - Prob. 3TYKCh. 4.4 - Prob. 1BCCh. 4.4 - Prob. 1CCCh. 4.4 - Prob. 1TYKCh. 4.4 - Prob. 2BCCh. 4.4 - Prob. 2TYKCh. 4.4 - Prob. 3TYKCh. 4.5 - What is the function of the nuclear lamina and the...Ch. 4.5 - Prob. 1BCCh. 4.5 - Prob. 2CCCh. 4.5 - Prob. 1TYKCh. 4.5 - Prob. 2TYKCh. 4.5 - Prob. 3TYKCh. 4.6 - What is the advantage of having a highly...Ch. 4.6 - Prob. 1BCCh. 4.6 - Prob. 2CCCh. 4.6 - Prob. 1TYKCh. 4.6 - Prob. 2TYKCh. 4.7 - Prob. 1TYKCh. 4.8 - Prob. 1CCCh. 4.8 - Prob. 2CCCh. 4.8 - Prob. 3CCCh. 4.8 - Prob. 4CCCh. 4.8 - Prob. 1TYKCh. 4.8 - Prob. 2TYKCh. 4.8 - Prob. 3TYKCh. 4.9 - Which of the following could be found in a...Ch. 4.9 - Prob. 2TYKCh. 4 - The cell theory states that all living things are...Ch. 4 - Prob. 2TYCh. 4 - Prob. 3TYCh. 4 - Prob. 4TYCh. 4 - Prob. 5TYCh. 4 - Prob. 6TYCh. 4 - Prob. 7TYCh. 4 - Prob. 8TYCh. 4 - Prob. 9TYCh. 4 - Prob. 10TYCh. 4 - Describe two specific ways that protein-protein...Ch. 4 - Explain how motor proteins and cytoskeletal...Ch. 4 - A principle of biology is that structure...Ch. 4 - Prob. 1CBQCh. 4 - Prob. 2CBQ
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- Figure 15.13 Errors in splicing are implicated in cancers and other human diseases. What kinds of mutations might lead to splicing errors? Think of different possible outcomes if splicing errors occur.arrow_forward. In bacterial genes, as soon as any partial mRNA transcriptis produced by the RNA polymerase system, the ribosome assembles on it and starts translating. Draw a diagram of this process, identifying 5′ and 3′ ends of mRNA,the COOH and NH2 ends of the protein, the RNA polymerase, and at least one ribosome. Why couldn’t this system work in eukaryotes?arrow_forwardCan I have a detailed explanation on how alternative splicing might affect metabolism. Thank you!arrow_forward
- You are a research scientist working in genetic engineering. You create a piece of DNA that you want to express in E. coli, a prokaryote. This piece of DNA (represented by the schematic in the figure) consists of a bacterial promoter, a ribosome binding sequence (RBS), a eukaryotic gene and a terminator sequence. Do you think that this piece of DNA would be expressed if placed into an E. coli cell that contains all the machinery needed for gene expression? Fully motivate your answer.arrow_forward1)A. how do you read a sequence of DNA (template or non-template strand) to convert it an mRNA sequence and to a protein? B.How does chromatin remodeling regulate gene transcription? C. What are the major differences between gene expression in bacteria and eukaryotes D. How are non-coding regions involved in gene transcription? E. Explain how eukaryotic genes sometimes produce multiple protein products?arrow_forwardYou are a research scientist working in genetic engineering. You create a piece of DNA that you want to express in a mouse cell, which is a eukaryote. This piece of DNA (represented by the schematic in the figure) consists of a eukaryotic promoter, a prokaryotic gene lacking introns and exons and a eukaryotic terminator sequence. Do you think that this piece of DNA would be successfully expressed if placed into a mouse cell containing all the machinery needed for gene expression? Fully motivate your answer. The AAUAAA Eukaryotic Start Coding Stop terminator promoter codon sequence codon sequencearrow_forward
- Consider how alternative splicing could allow many different protiens to be encoded by the same gene?arrow_forward. Let’s say that you have incredible skill and can isolate the white and red patches of tissue from the Drosophila eyes shown in Figure 12-24 in order to isolate mRNA from each tissue preparation. Using your knowledge of DNA techniques from Chapter 10, design an experiment that would allow you to determine whether RNA is transcribed from the white gene in the red tissue or the whitetissue or both. If you need it, you have access to radioactive white-gene DNAarrow_forwardWhich of the following methods is utilized by eukaryotes to control their gene expression that is different from the type of control found in bacteria? a) control of chromatic remodeling. b) control of RNA splicing. c) transcriptional control. d) control of both RNA splicing and chromatin remodeling.arrow_forward
- Which of the following is likely associated with the mRNA processing step of alternative splicing? 1. A gene is discovered to encode for two different proteins one protein has 46 amino acids and the other protein has 86 amino acid 2. A gene has 7 exons. one processed mRNA transcript from this gene contains exons 3, 4, 6 and 7. a second process mRNA transcript from this gene contains exons 2, 3, 5, and 7 3. The open reading frame of a gene contains 90 nucleotides but the translated polypeptide contains only 29 amino acids 4. Complexity of the transcrptome ( # of different mRNA molecules) increasesarrow_forwardResearchers are designing and testing antisense drugs as therapies for a variety of diseases, inclusing cancer, AIDS, diabeates, and muscular dystrophy. The drugs are also being tested to fight infection by deadly viruses such as Ebola. Antisense drugs consist of short RNA strands coplementary in sequence to mRNAs that form during the progression of a disease. How do you think these drugs work?arrow_forward5. A mutant strain of Salmonella bacteria carries a mutation of the rho protein t hat has full activity at 37°C but is completely inactivated when the mutant strain is grown at 40°C. (Question # 21; Chapter 8-Genetics: An Integrated Approach). Speculate about the kind of differences you would expect to see if you compared a broad spectrum of mRNAs from the mutant strain grown at 37°C and the same spectrum of mRNAs from the strain when grown at 40°C. Are all mRNAs affected by the rho protein mutation in the same way? Why or why not?arrow_forward
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