Microbiology: An Introduction
12th Edition
ISBN: 9780321929150
Author: Gerard J. Tortora, Berdell R. Funke, Christine L. Case
Publisher: PEARSON
expand_more
expand_more
format_list_bulleted
Concept explainers
Textbook Question
Chapter 5, Problem 7MCQ
Which culture produces the most lactic acid?
Use the following choices to answer questions.
- a. E. coli growing in glucose broth at 35°C with O2 for 5 days
- b. E. coli growing in glucose broth at 35°C without O2 for 5 days
- c. both a and b
- d. neither a nor b
Expert Solution & Answer
Want to see the full answer?
Check out a sample textbook solutionStudents have asked these similar questions
Which of the following results from TSI test is FALSE?
A. Yellow slant, yellow butt, split agar means glucose, lactose and/or sucrose fermented gas produced
B. Yellow butt, red slant means glucose only fermented
C. Black slant, red butt means peptones were catabolized
D. Red slant, black butt means glucose fermented hydrogen sulfide gas produced
E. Red butt, red slant means all sugars fermented, no gas produced
t /g = (Log Nt – Log N0) /0.301
I introduce a loopful of Escherichia coli cells (say, 1000) into 10 mL of Nutrient Broth at 8 p.m. the night before your lab. The cells were taken from a culture plate (Nutrient Agar) held at 37°C, and inoculated into broth at the same temperature. They were held at 37°C overnight in a shaking water bath. At what time would the culture reach the Stationary Phase? Recall that doubling time under optimal conditions (these are) is 20 minutes.
A growing bacterial culture has 10,000 CFU/mL at noon and 10,000,000 CFU/mL at 6 p.m. What is the generation time under these conditions? What are your assumptions?
At midnight you inoculate 10 mL of a culture of Enterococcus with 103 cells/mL into 990 mL of the same medium, held under the same conditions as the original culture. At what time would the culture reach 107 cells/mL? Assume exponential growth over the period. Assume that g=half an hour. Note: We worked a different variant of this problem in…
Lysine decarboxylase media is yellow after incubating an organism in it at 37 degrees for 24 hours. Which of the following can be interpreted from this result?
A.
The organism did not grow so lysine decarboxylase activity could not be determined.
B.
The organism is negative for lysine decarboxylase.
C.
The media is too acidic to determine lysine decarboxylase activity.
D.
The organism is positive for lysine decarboxylase
Chapter 5 Solutions
Microbiology: An Introduction
Ch. 5 - Prob. 1RCh. 5 - DRAW ITUsing the diagrams below, show each of the...Ch. 5 - DRAW IT An enzyme and substrate are combined. The...Ch. 5 - Define oxidation-reduction, and differentiate the...Ch. 5 - There are three mechanisms for the phosphorylation...Ch. 5 - All of the energy-producing biochemical reactions...Ch. 5 - Fill in the following table with the carbon source...Ch. 5 - Write your own definition of the chemiosmotic...Ch. 5 - Why must NADH be reoxidized? How does this happen...Ch. 5 - NAME IT What nutritional type is a colorless...
Ch. 5 - Which substance in the following reaction is being...Ch. 5 - Which of the following reactions produces the most...Ch. 5 - Prob. 3MCQCh. 5 - Which of the following compounds has the greatest...Ch. 5 - Prob. 5MCQCh. 5 - Prob. 6MCQCh. 5 - Which culture produces the most lactic acid? Use...Ch. 5 - Which culture produces the most ATP? Use the...Ch. 5 - Which culture uses NAD+? Use the following choices...Ch. 5 - Which culture uses the most glucose? Use the...Ch. 5 - Explain why, even under ideal conditions,...Ch. 5 - The following graph shows the normal rate of...Ch. 5 - Compare and contrast carbohydrate catabolism and...Ch. 5 - How much ATP could be obtained from the complete...Ch. 5 - The chemoautotroph Acidithiobacillus can obtain...Ch. 5 - Haemophilus influenzae requires hemin (X factor)...Ch. 5 - The drug Hivid, also called ddC, inhibits DNA...Ch. 5 - The bacterial enzyme streptokinase is used to...
Additional Science Textbook Solutions
Find more solutions based on key concepts
Modified True/False 6. __________ Halophiles inhabit extremely saline habitats, such as the Great Salt Lake.
Microbiology with Diseases by Body System (4th Edition)
Write an equation that uses the products of photosynthesis as reactants and the reactants of photosynthesis as ...
Campbell Biology in Focus
Explain how biosensors might be used for water quality testing. Would biosensors give more accurate identificat...
Microbiology with Diseases by Taxonomy (5th Edition)
QUANTITATIVE Punnett Squares as Genetic Tools. The genetic characters of seed color (where Y is the allele for ...
Becker's World of the Cell (9th Edition)
In Drosophila, a cross was made between femalesall expressing the three X-linked recessive traits scute bristle...
Concepts of Genetics (12th Edition)
All of the following processes are involved in the carbon cycle except: a. photosynthesis b. cell respiration c...
Human Biology: Concepts and Current Issues (8th Edition)
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- Answer the following. A. Which part of the refrigerator should be used for storing raw meat? B. How long can raw meat stay in fridge? C. At what temperature should meat be refrigerated?arrow_forwardWhich type of PAGE would you use for the following? a.Monitoring the hydrolysis of casein in milk by plasmin b. Monitoring changes in meat proteins during aging of meat. c.Investigating the Protein profile of rice protein concentrate from different sources.arrow_forwardIf the inoculated lactose broth cube exhibits acid production but without gas formation, will you consider this as a negative or positive result? Explain Why is the MPN test qualitative rather than quantitative? Why is the MPN test qualitative rather than quantitative? Why is MPN test qualitative rather than quantitativearrow_forward
- The table shows the composition of Nutrient Agar per liter.Suppose that you need to prepare 700 mL of Nutrient Agar. How much (in g) of each component do you need? a. Beef extract b. Sodium Chloride c. Agar d. Peptonearrow_forwardThe diagram shows the growth curve of Escherichia coli in a suspension culture containing glucose and lactose. Optical density is used to measure the concentration of bacteria in the suspension culture. Use the image to answer the question. Which statement BEST supports the information in the graph? A. E. coli metabolizes lactose and glucose. B. E. coli metabolizes glucose first, then lactose. C. E. coli metabolizes lactose first, then glucose. D. E. coli metabolizes lactose and glucose at the same time.arrow_forwardWhich is an efectrogenic pump? Select one: O A. sucrose cotransporter O B. Both Na+/K+ pump and H+ pump O C. glucose pump O D. Na+/K+ pump O E. H+ pumparrow_forward
- Describe how each of the following would appear when grown on EMB agar: a. Escherichia coli b. Pseudomonas aeruginosarrow_forwardIn this lab, we used three different bacteria (E.Coli, B. Subtilis, and A. Faecalis) in fermentation assays to determine their ability to ferment three different carbohydrates: lactose, glucose and sucrose. In the table below record the results of the experiment using "P" for positive and "N" for negative results.arrow_forwardYour nasal sample showed colonies with a clear halo around them on blood agar. What can you determine based on that observation? a. The organism is a mannitol fermenter b. The organism is able to utilize starch c. The organism is reducing the hemoglobin and damaging the erythrocytes d. The organism is beta-hemolytic e. The organism is unable to use red blood cells as a nutrient sourcearrow_forward
- Menaquinones is produced by Flavobacterium meningosepticum via submerged fermentation with 60% working volume in 30-L fermentation. To obtain the crude menaquinones, the fermentation medium is centrifuged at 12,000 rpm for 15 minutes. The vacuum-freeze dried particles are crushed and collected. After that the samples are treated with methanol for 20 minutes. To remove polar lipid compounds from bacterial cells, HZ816 macroporous adsorption resin column is used with methanol-dichloromethane (l:1) as mobile phase and flow rate of 0.75 mL/min. Rotary evaporation at 500 C is carried out to concentrate the eluent. After that, the concentrated sample is purified with Bio-BeadsTM S-X3 to remove components without UV absorption. To obtain the homolog purified menaquinones, RP-C18 column is used. Recrystallization is carried out to obtain pure crystalized menaquinones. Answer the following: Indicate how to obtain the sample in the methanol solvent. Three different chromatography are used in…arrow_forwardThermal images are photographs of the heat emittedby an object. Researchers have used thermal imaging ofplants to isolate mutants that overproduce abscisic acid.Suggest a reason why they are warmer than wild-typeplants under conditions that are normally nonstressful.arrow_forwardDescribe what the plate in each scenario looks like: A. Bacteria grows on mannitol salt agar and is negative for mannitol fermentation B. Bacteria grows on EMB agar and is positive for lactose fermentation C. Bacteria grows on MacConkey agar and is negative for lactose fermentationarrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
cell culture and growth media for Microbiology; Author: Scientist Cindy;https://www.youtube.com/watch?v=EjnQ3peWRek;License: Standard YouTube License, CC-BY