Bio 370 Paper 3 Worksheet
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Biology 370
Principles of Muscle Physiology & Energetics
Paper Discussion 3 Worksheet
Participant Names (max 5):
Brand MD, Turner N, Ocloo A, Else PL & Hulbert AJ. 2003. Proton conductance and fatty
acyl composition of liver mitochondria correlates with body mass in birds.
Biochemistry
Journal
. 376: 741-748.
In preparation for class, please read the above referenced paper, which can be found in
the Module 10 on Canvas.
Due Date:
completed worksheet is due on November 16
th
, 2023 at 8 p.m. on Canvas
Question 1. (3 marks)
a)
Provide one reason
why mitochondrial proton leak
is potentially
detrimental
to
metabolism.
Explain why it is detrimental.
(1 mark)
b)
All mitochondria show some degree of proton leak, suggesting that it may have a
physiological purpose.
Propose
one potential physiological benefit
to mitochondrial
proton leak and explain why it would be beneficial.
(1 mark)
c)
Why do you think the mitochondria from ectotherms generally have low proton leak?
(1 mark)
Question 2. (3 marks)
This paper states a goal for the study in the last paragraph of the introduction, but the
authors did not frame their investigation around a clearly articulated hypothesis and they
didn’t provide specific predictions for their study.
Please provide a
posteriori
(after the
fact) hypothesis for this study and at least two predictions.
“In the present study, we analysed the relationship between phospholipid
fatty acyl
composition of liver mitochondria
,
mitochondrial proton conductance
and body
mass
in
birds to investigate whether the allometric relationships found in mammals are general for
all endotherms, or specifically mammalian.”
Hypotheses
(1 mark)
:
1
Predictions
(2 marks)
:
Question 3. (2 marks)
Oligomycin is a selective inhibitor of the F
1
F
O
-ATP synthase (aka complex V of the electron
transport system) and is used to help address the goals of their study.
How does the use
of oligomycin help the authors address their study goals?
(1 mark)
What unique data are
generated through the use of this drug that are important to addressing the study
objectives.
(1 mark)
Question 4. (2 marks)
Figure 2
shows the ratios of different
mitochondrial respiration rates (respiratory control
ratios)
relative to the
body mass
of the birds under investigation.
What do the data
presented in Figure 2 reveal about the relationship
between mitochondrial function and
body mass
?
Question 5. (2 marks)
The mitochondrial respiration data presented along the y-axis of Figure 4 (a and b) is
presented at a constant membrane potential (170 mV) across the inner mitochondrial
membrane.
Why is it important to do this in the context of assessing proton leak.
Question 6. (2 marks)
Briefly describe using non-expert language how the data presented in Figure 5 relates to,
and possibly explains, the data presented in Figure 4b.
Total:
/14
2
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