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Germination Lab

Decent Essays

Aim:
Does adding a Willow solution affect the germination of mustard seeds?
Ho/null hypothesis- The Willow won’t affect the germination of mustard seeds.
Ha/alternative hypothesis- The Willow will affect the germination of the mustard seeds.

Method:
For trial 1: first I used the Willow to make up a solution, I did this by breaking up the Willow and putting it in a pot of water, then boiled it. After the solution was ready, I counted out 200 mustard seeds and placed 100 of them on a tissue and the other 100 on another tissue. Then I used an eye dropper and placed 100 drops of water on one tissue and 100 drops of Willow solution to the other tissue (until the tissue was soaked). After 24 hours I checked the mustards seeds, and counted how many …show more content…

After 24 hours, the tissue had mostly dried out, so I added 20 more drops of water to the water tissue and 20 drops of Willow solution to the Willow tissue. I then checked the seeds again after 52 hours.

Results:
The first experiment showed after 24 hours that the tissue with just water, had 89% of the seeds had germinated, and the tissue with Willow solution the seeds germinated at 58%. It was also observed that the seeds that had germinated with the water had also grown more than the germinated seeds in the Willow solution.

Trial 1 Without Willow With Willow
Germination after 24 hours (%) 89% 58%

The second experiment showed that the tissue with water germinated at 90%, where the tissue with the Willow solution germinated at 56%, after 24 hours. The observations at 24 hours for both experiments were very similar. Then, after 52 hours, the seeds from the water tissue had 97% germinated and the seeds from the Willow tissue germinated at 96%. Also, between the first time I checked and the second, the seeds with the Willow solution grew faster and were now mostly longer than the germinated seeds with the water.

Trial 2 Without Willow With Willow
Germination after 24 hours (%) 90% 56%
Germination after 52 hours (%) 97% …show more content…

For a seed to germinate, it needs to take in water, the water makes the embyo larger and longer. In seeds there are protein storages, like globulins, and as the seed gets older, more of these protein storages are produced. Slowed germination rate (Trials 1 and 2, before at 24 hours for Willow) may have be caused by the salinity (saltiness), as this can alter the amount of nitrogen in the seed (Miransari, M, 2013). ABA also inhibits germination, and for germination to occur there needs to be a decrease in the amount of ABA during the stage of imbibition (the absorption of solvent by a gel), and phase II. The inhibition of germination caused by ABA, can be reversed by gibberellins and ethylene (Weithrecht, K & Muller, K & Leubner-Metzger, G, 2011). Usually there is not a lot of ABA present in a seed during the early stages, and the amount of ABA increases in the middle stages (Bewley, J, 1997). So the salinity I do not think is likely to have been what caused the decreased germination rate, because if the willow was too salty it’s growth would have been slowed too (which it could have been, I do not know for sure). However, the two plants may require different salinity levels to grow, so that could have affected the germination rate. The germination rate could also have

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