1. 100 μl is equal to 2. 10 μg is equal to ng and mg/ml 3. 70 μg/ml is equal to ng/μl and mg/ml. 4. Suppose you have a DNA solution that is 100 ng/μl, and you want to put 0.01 mg of DNA into a How many μl of your DNA solution should you use? 5. Show the procedure & calculations for preparing 10-3x and 10-5x dilutions of a 1x 6. How much agarose do you need for a 1.5% gel? 7. Show all the calculations for a complete restriction digest. For example, suppose you have a DNA solution at 400 ng/μl and an EcoRI solution at 20 Units/μl, and you want to cut 2 µg of DNA in a small reaction volume. What do you do? You need to know what goes into a restriction digestion reaction (all the reagents), and the final reaction volume (most often you will be given this), perform calculations for each reagent and place them in a TABLE (which should include WATER and the TOTAL volume) 8. Suppose you just did the restriction digest described above. What is the concentration of DNA in your digest? If you want to perform a ligation using 800 ng of your cut DNA, how many μl should you use? 9. Suppose the restriction digest cut your 1-kb DNA into three fragments of 200 bp, 300 bp, and 500 bp. You run 1 µg of cut DNA on the How much DNA is in each of the bands? Think carefully here.
1. 100 μl is equal to 2. 10 μg is equal to ng and mg/ml 3. 70 μg/ml is equal to ng/μl and mg/ml. 4. Suppose you have a DNA solution that is 100 ng/μl, and you want to put 0.01 mg of DNA into a How many μl of your DNA solution should you use? 5. Show the procedure & calculations for preparing 10-3x and 10-5x dilutions of a 1x 6. How much agarose do you need for a 1.5% gel? 7. Show all the calculations for a complete restriction digest. For example, suppose you have a DNA solution at 400 ng/μl and an EcoRI solution at 20 Units/μl, and you want to cut 2 µg of DNA in a small reaction volume. What do you do? You need to know what goes into a restriction digestion reaction (all the reagents), and the final reaction volume (most often you will be given this), perform calculations for each reagent and place them in a TABLE (which should include WATER and the TOTAL volume) 8. Suppose you just did the restriction digest described above. What is the concentration of DNA in your digest? If you want to perform a ligation using 800 ng of your cut DNA, how many μl should you use? 9. Suppose the restriction digest cut your 1-kb DNA into three fragments of 200 bp, 300 bp, and 500 bp. You run 1 µg of cut DNA on the How much DNA is in each of the bands? Think carefully here.
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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solve this with step by step calculations for every question please
Transcribed Image Text:1. 100 μl is equal to
2. 10 μg is equal to ng and mg/ml
3. 70 μg/ml is equal to ng/μl and mg/ml.
4. Suppose you have a DNA solution that is 100 ng/μl, and you want to put
0.01 mg of DNA into a How many μl of your DNA solution should you use?
5. Show the procedure & calculations for preparing 10-3x and 10-5x dilutions
of a 1x
6. How much agarose do you need for a 1.5% gel?
7. Show all the calculations for a complete restriction digest. For example,
suppose you have a DNA solution at 400 ng/μl and an EcoRI solution at
20 Units/μl, and you want to cut 2 µg of DNA in a small reaction volume.
What do you do? You need to know what goes into a restriction digestion
reaction (all the reagents), and the final reaction volume (most often you
will be given this), perform calculations for each reagent and place them in
a TABLE (which should include WATER and the TOTAL volume)
8. Suppose you just did the restriction digest described above. What is the
concentration of DNA in your digest? If you want to perform a ligation
using 800 ng of your cut DNA, how many μl should you use?
9. Suppose the restriction digest cut your 1-kb DNA into three fragments of
200 bp, 300 bp, and 500 bp. You run 1 µg of cut DNA on the How much
DNA is in each of the bands? Think carefully here.
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