A 500. µl aliquot of a caffeine unknown is diluted to a total volume of 10.00 mL. A calibration curve for caffeine was generated using external standards on the HPLC. The equation of the curve Caffeine Area(mV cm)=1.266C(ppm)+0.029. If the HPLC peak for the diluted unknown has an area of 3.07 mV cm, what is the original caffeine concentration in ppm?

Appl Of Ms Excel In Analytical Chemistry
2nd Edition
ISBN:9781285686691
Author:Crouch
Publisher:Crouch
Chapter14: Chromatography
Section: Chapter Questions
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A 500. µl aliquot of a caffeine unknown is diluted to a total volume of 10.00 mL.
A calibration curve for caffeine was generated using external standards on the HPLC. The
equation of the curve
Caffeine Area(mV cm)=1.266C(ppm)+0.029.
If the HPLC peak for the diluted unknown has an area of 3.07 mV cm, whąt is the original
caffeine concentration in ppm?
Transcribed Image Text:A 500. µl aliquot of a caffeine unknown is diluted to a total volume of 10.00 mL. A calibration curve for caffeine was generated using external standards on the HPLC. The equation of the curve Caffeine Area(mV cm)=1.266C(ppm)+0.029. If the HPLC peak for the diluted unknown has an area of 3.07 mV cm, whąt is the original caffeine concentration in ppm?
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