The calcium ion binds to a certain protein to form a 1:1 complex. The following data was obtained in an experiment: Total Ca2+/uM 60 120 180 240 480 Ca2+ bound to Protein/uM 31.2 51.2 63.4 70.8 83.4 Determine graphically the dissociation constant of the Ca2+ - protein complex. The protein concentration was kept at 96uM for each run. (1uM = 1 x 10-6 M.)

The calcium ion binds to a certain protein to form a 1:1 complex. The following data was obtained in an experiment: Total Ca2+/uM 60 120 180 240 480 Ca2+ bound to Protein/uM 31.2 51.2 63.4 70.8 83.4 Determine graphically the dissociation constant of the Ca2+ - protein complex. The protein concentration was kept at 96uM for each run. (1uM = 1 x 10-6 M.)

Principles of Modern Chemistry

8th Edition

ISBN:9781305079113

Author:David W. Oxtoby, H. Pat Gillis, Laurie J. Butler

Publisher:David W. Oxtoby, H. Pat Gillis, Laurie J. Butler

Chapter20: Molecular Spectroscopy And Photochemistry

Section: Chapter Questions

Problem 67CP

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The calcium ion binds to a certain protein to form a 1:1 complex. The following data were obtained in an experiment. Total Ca2+/µM 60.9 122 186 250. 536 Ca2+ bound to Protein/µM 31.4 51.4 63.6 71.0 83.6 Determine graphically the dissociation constant of the Ca2+-protein complex. The protein concentration was kept at 97 µM for each run. (1 µM = 1×10-6 M.) Kd = 110
Construct the expression for Ksp for solid A9CN in aqueous solution. AGCN(s) = Ag*(aq) + CN-(aq) 1 Based on your knowledge of how the solid will dissociate in aqueous solution, use the tiles to form the expression. Ksp RESET [AGCN] 2[A9CN] [A9CNJ? [Ag*] 2[Ag*] [Ag*P [Ag*] 2[Ag*] [Ag*P [CN] 2[CN] [CN-P [CN²] 2[CN ] [CN-1?
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