This virtual HHMI virtual immunology lab was testing for lupus. How is this same test used to test for the presence of Covid-19? If the results of a COVID-19 test were the same as in this exercise, what would they indicate about the three patients?
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This virtual HHMI virtual immunology lab was testing for lupus. How is this same test used to test for the presence of Covid-19? If the results of a COVID-19 test were the same as in this exercise, what would they indicate about the three patients?
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- This virtual lab was testing for lupus. How is this same test used to test for the presence of HIV? If the results for an HIV test were the same as in this exercise, what would they indicate about the three patients?A COVID-19 test, which produce result in 10 minutes, has the following test characteristics. The test has a 95% sensitivity and a 95% specificity. The State of Massachusetts is in dilemma whether to test symptomatic individuals or the entire residents to decrease the spread of infection as depicted in the following two scenarios. Scenario A. The State of Massachusetts wants to administer the test on 40,000 individuals who will develop cough, fever and shortness of breath in the next 10 days to identify COVID-19 infected individuals and institute public health measures. The estimated prevalence of COVID-19 in this population is 750 per 1,000 population using a gold-standard test. Scenario B. The State of Massachusetts wants to administer the test to its entire COVID-19 uninfected population (6,750,000). The estimated prevalence of COVID-19 in this population is 5 per 1,000 population using a gold-standard test. Please help the State of Massachusetts by responding to the…The plaque assay plate below was made from a dilution of 10-7 and 0.1 ml of the dilution was plated on the cell monolayer. What is the virus titer in PFU/ml? Note: 10e1 means 10 to the first power, etc. Select all answers that apply.
- To diagnosis, we used a procedure known as a "sandwich ELISA" (Enzyme-Linked Immunosorbent Assay). 1.In the "ELISA sandwich," what is the "meat"? 2.Both patients A and B had positive results. Why are these two patients' outcomes so different? 3.What is one potential cause of an ELISA false negative result? What is one potential cause of an ELISA false positive result? What is another example of how an ELISA assay should be used?There are many immunotherapeutics now being used to immunomodulate recipients that are either fully human monoclonal antibodies or partially humanized monoclonal antibodies. Discuss whether or not they may interfere with solid phase antibody assays, CDC assays or cell based flow cytometry crossmatches.How reliable are Lateral flowimmunochromatographic assays when compared to molecular diagnostic tests or other serological tests? Please discuss referring to original articles example of covid 19..
- For an Immunoprecipitation experiment: What is the function of the IP-2 tube in this experiment?Many ELISAs that are used to identify the presence of antibody to viruses have a 3rd control (along with negative and positive). In between the rows of wells coated with antigen, are rows of cells coated with tissue culture used to grow the virus. In other words, patient serum is added to wells that contain antigen along with wells that contain tissue culture. Why do you think this might be necessary?As everyone is aware of, suspected COVID-19 patients are being diagnosed using the Nasopharyngeal Swab Test through Reverse-Transcriptase Polymerase Chain Reaction. Explain its principle; its advantages and disadvantages.
- If you wanted to make an assay detecting SARS-CoV-2 virus presence, like the assay shown in the previous question, what would be a logical combination of things to include in your list of supplies to obtain? rabbit anti-SARS-cov2 spike antibody, anti-rabbit IgG horseradish peroxidase-conjugated antibody, tetramethylbenzidine rabbit anti-SARS-cov2 spike antibody, anti-mouse horseradish peroxidase conjugated antibody, tetramethylbenzidine rabbit anti-SARS-cov2 spike antibody, anti-rabbit SARS-cov2 spike antibody, tetramethylbenzidine SARS-cov2 spike, anti-rabbit IgG horseradish peroxidase-conjugated antibody, tetramethylbenzidine rabbit anti-SARS-cov2 spike horseradish peroxidase conjugated antibody, anti-rabbit horseradish peroxidase conjugated antibody, tetramethylbenzidineWhat are the differences between “O” and “H” antigen? Differentiate Widal from Weil-Felix test. The strains of Proteus vulgaris are used in Weil-felix reaction. Explain why.If PBR323 is used instead of PUC18, is blue white screening applicable? Justify your answer.