Biology
Biology
5th Edition
ISBN: 9781260487947
Author: BROOKER
Publisher: MCG
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Chapter 21.5, Problem 1CS
Summary Introduction

To propose: A model that can determine the outcome of transposition depending on the pattern of replication.

Introduction: DNA is a type of nucleic acids that is found in living organisms. DNA stands for “Deoxyribonucleic acid.” The process of the formation of daughter strands from DNA strands is termed as the replication of DNA. The enzyme RNA primer initiates the synthesis of daughter DNA strands from the template strand. The RNA primer is synthesized by an enzyme known as DNA primase. Two strands are synthesized by this enzyme; one is the leading strand, while the other is the lagging strand.

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DNA Replication Drawing Name: Using penci, you will draw a representation of DNA replication along the leading and lagging strands. Follow the directions below, drawing each element in its proper location along the replicating DNA strand. Once you are sure everything is in the correct place, complete your drawing by adding color to distinguish objects as separate. 1. On the diagram below, label the 5 and 3' onds of both parental DNA strands (you can make up which is which) 2 Label the replication fork 3. Draw and label helicase 4. Label the overall direction of DNA replication 5. Draw and label single stranded binding proteins 6. Draw and label the leadng strand 7. Draw and label a single DNA polymerase IIl on the leading strand 8. Draw and label an RNA primer on the leading strand 9. Draw and label a DNA polymerase I on the leading strand 10. On the lagging strand, draw and label at least three Okazaki fragments 11. On the lagging strand. draw and label at least two DNA polymerase IIl…
a. Propose three different mutations to prevent initiation, elongation, and termination of bacterial DNA replication, respectively. Explain how/why each mutation would prevent its respective step. (Hint: mutations can be in genes that encode proteins or regulatory DNA sequences) b. In the early 1900s, Avery, MacLeod, and McCarty performed an experiment in bacterial cells to determine whether DNA, RNA, or protein functions as the 'transforming molecule' (i.e. the genetic material). In your own words, how did their experiment (depicted in the figure below) help to answer that question?
Home Work: • Suppose you perform a PCR that begins with one double-strand of the following DNA template: +5' -СТАССТСCGGGTTGACTGСТАССТТССССGGATGCCCAAAAТТСТСGAG-3— :::::::::::: :::::::::::: :::: +3'-GATGGACССССААСТGACGATGGAAGGGCCCТАССGGTTTTAAGAGCTC-5'+ A. Draw one cycle of PCR reaction below the following diagram. B. Label the template DNA, the primers, and what is happening at each step. (1) température cycle #1
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