Concept explainers
To describe: The reason for using two culture tubes for analysis.
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Explanation of Solution
Two culture tubes are required for the microbial analysis. One culture tube is used to identify and view the particular microbes of interest by following the specific microscopical and staining techniques. The other culture tube is used for performing the other serological analysis that includes biochemical and immunological assays. Having two culture tubes would provide sufficient availability of the sample specimens for analysis with minimal possibilities of contamination.
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Chapter 3 Solutions
Microbiology for Surgical Technologists (MindTap Course List)
- A surgical technology student is completing rotation in the central sterile processing department. The student has been asked to assemble a dilatation and curettage (D&C) tray from the racks of sterilized instruments. How will the student know what instruments this facility routinely puts in the D&C instrument tray?arrow_forwardSubject : Environmental Microbiology Can u use the information given below to answer these 2 question 1.Provide an aim for this lab 2. Provide objectives DISCUSSION QUESTIONS What is the relationship between the resolution power and the useful magnification that may be obtained with the light microscope? What determines the resolving power of the lens system? What is the limit of resolution obtainable with the light microscope? How you will distinguish between bright field and dark-field microscopy and provide a specific example where each would be method of choice for observing a culture of bacteria? What advantages does electron microscopy have over light microscopy? What are disadvantages of electron microscopy over light microscopy? #Compare the use and the methodology of TEM with SEM? Provide at least one example where each would be the method of choicarrow_forwardBriefly discuss 2 advantages and 2 disadvantages of using the paraffin embedding method for histological examination of tissues as opposed to the frozen techniquearrow_forward
- Discusses the methods to -1 optimise fluorescent cellular (staining (priority first List the freezing -2 parameters of the slow cooling technique Parameter of verification -3 precedure of freezing 9arrow_forwardThis is a typical lab exam question. (Problem-solving, uses current topic.)Your TA performed the antibiotic disc experiment using supplies from their research lab. The antibiotic discs had the year 2002 written on their containers.Your TA grew a pure culture of unknown bacteria. They applied the culture to a fresh agar plate and applied the 2002 antibiotic discs to the plate and incubated it for growth. They found that the mystery bacteria species was resistant to all three antibiotic discs that were applied to the plate.Are there any control groups that could be used to confirm or refute this multi-drug resistance? Describe them.arrow_forwardsite your reference:) What are the basic concepts and precautions to be observed in specimen collection for Microbiological examination? Enumerate specific examples of clinical specimens collected for microbiological examination. Give 2 specific examples of bacteria isolated from clinical samples. What is a transport medium; give specific examples and state their purpose.arrow_forward
- Give the uses/functions and images of each apparatuses. Basic Laboratory Equipment Uses/Functions Picture 1. Microscope 2. Colony Counter 3. Autoclave 4. Microbiology incubator 5. Drying oven 6. Refrigerator (microbiology) 7. Bunsen burner/alcohol lamp 8. Candle jar 9. Anaerobic jar 10. Microhood or Bacteriologic hood/Safety hood/Safety cabinet 11. Bacteriologic filters (Seitz, Chamberlain, Berkfield) 12. Petri dish 13. Culture tubes 14. Hanging drop slide 15. Durham’s tube 16. Staining rack 17. Thermostatically controlled water bath 18. Inoculating loop 19. Inoculating needle 20. Vialsarrow_forwardPlease match the test with its description to assess your understanding of the kinds of data collected during information gathering and your understanding of the processes involved in identifying microbes from samples. 1. using macroscopic and microscopic traits for identification appearance 2. tests that determine chemical characteristics including enzyme production and nutritional requirements of the microbe biochemical tests 3. analyzing the genotype of an organism DNA profiles 4. tests the organism against known antibodies to determine if there is a reaction between the organism and the antibody immunologic testing varrow_forwardHow would you draw out a step in an experiment that included the following? Please help, I am so lost. Metal coupon inoculation (vortex/bead method) and assessment of viable cells by culture to simulate wet fomite or dry touch surface contamination.arrow_forward
- Questions 14-16 are based on the following. In the 1940's, Avery, Macleod, and McCarty transformed nonencapsulated bacteria into encapsulated. forms by growing the nonencapsulated cells in a culture containing an extract made from dead encapsulated cells. The transformed cells produced colonies of encapsulated bacteria. Three different procedures and their results are outlined below. Procedure I: Extract made from dead encapsulated cells added to culture medium. Nonencapsulated bacteria added to culture medium. Results: Both nonencapsulated and encapsulated bacteria grow. Procedure II: Extract made from dead encapsulated cells treated with protein-degrading enzymes before adding extract to culture medium. Nonencapsulated bacteria added to culture medium. Results: Both nonencapsulated and encapsulated bacteria grow. Procedure III: Extract made from dead encapsulated cells treated with DNAse (an enzyme that selectively destroys DNA) before adding extract to culture medium.…arrow_forward(1) why can't we say "sterile" technique (2) how are aseptic technique similar and different in the lab and Healthcare field?Be specific and explain at least 2 differences and two similarities. (3) You are asked to develop a method of transfer an unknown organism from a liquid broth to a solid petri dish.list each step that you would have to take .be specificarrow_forwardDiscarding dirty, used micropipette tips is one way to practice sterile technique. What are some other ways to practice sterile techique in lab this week? Check all that apply. Wear gloves while working with the EcoPlate, micropipette, and soil serial dilutions. Leave your sterile tubes uncapped and open, for airborne bacteria to enter. Keep food and drink off your lab bench. Remove your gloves before handling your computer, phone, etc. Use provided ethanol solution to clean your lab bench. Touch the soil or soil solutions with your bare hands or fingers.arrow_forward
- Microbiology for Surgical Technologists (MindTap ...BiologyISBN:9781111306663Author:Margaret Rodriguez, Paul PricePublisher:Cengage Learning