Picture that has dark background with red cells is the Negative stain and other picture in white is the Acid fast stain. so far this is the information I have: gram stain: positive motility: motile oxygen requirement: Facultative anaerobe and than I need these two acid fast stain: _____??? negative stain: _____????

Picture that has dark background with red cells is the Negative stain and other picture in white is the Acid fast stain. so far this is the information I have: gram stain: positive motility: motile oxygen requirement: Facultative anaerobe and than I need these two acid fast stain: _??? negative stain: _????

Basic Clinical Laboratory Techniques 6E

6th Edition

ISBN:9781133893943

Author:ESTRIDGE

Publisher:ESTRIDGE

Chapter7: Basic Clinical Microbiology

Section7.7: Bacterial Identification And Antibiotic Susceptibility Testing

Problem 2CS

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Picture in white is acid fast other picure is negative stain so far this is the information I have: gram stain: positive motility: motile oxygen requirement: Facultative anaerobe and than I need these two acid fast stain: _____??? negative stain: _____????
Why is it necessary to use a 24-hrold culture in gram staining? What does Gram staining reveal owing to its use in clinical diagnosis? Indicate color staining results of gram positive and negative bacteria for every hypothetical scenario. Modification in Procedure Staining results 1. Crystal violet was replaced with methylene blue. G+ G- 2. Mordant was skipped G+ G- 3. Decolorizing step was omitted G+ G- 4. counterstain was not used? G+ G- 5. Crystal violet and safranin was swapped in position. G+ G- What bacterial specimen represent the following: (a) positive simple staining; and (b) negative simple staining viewed under OIO. Provide details such as specimen and bacterial stain used and reference. Positive Staining 1000 X Negative Staining 1000 X
Is is best to observe slides of bacteria stained with the acid fast stain under high dry magnification. True or false
Out of twelve tests that can be performed: Gram Stain Wet Mount Endospore Staining Acid Fast Staining Differential & Selective Media (MacConkey, PEA, EMB, MSA Agar, Blood Agar, etc.) Oxidative-Fermentative Test Carbohydrate Fermentation MR-VP Testing Indole Production Citrate Utilization Triple Sugar Iron Testing Catalase Test. Which four tests are the best fit to help identify bacteria that is unknown? Possible bacterias that are being observed are: Enterobacter aerogenes Escherichia coli Micrococcus luteus Proteus vulgaris Pseudomonas aeruginosa Salmonella typhimurium Serratia marcescens Staphylococcus aureus Staphylococcus epidermidis
Which of the following are true about a differential stain. There may be more than one answer! No chemicals are needed. More than one stain is used The acid fast stain is an example The Gram stain is an example.
The figure attached shows the colonies on Eosin Methylene Blue Agar (EMBA) plate. Make a description on the picture.
Put the following Steps of gram smear in order Create a bacteria Add crystal violet to the bacterial smear. Rinse with water after 60 seconds. Allow the smear to air dry and then heat fix it. Add gram iodine to the smear, let it sit for 60 seconds and rinse water decolorize with 95% ethanolfoe 5-10 seconds and then rise with water Blot dry with bibulous paper Add safranin to the smear and let sit for 60 seconds, then rinse with water Observe using oil immersion
Explain why only gram-negative cells undergo decolorization during the gram staining procedure. Cite the purpose of each of the following reagents in a differential staining procedure. Primary stain Mordant Decolorizing Agent Counter stain What might happen if the Gram staining procedure is performed on a culture incubated for a little over a day
Imagine that you are Gram staining a mixed culture of both gram-positive and gram-negative bacteria. If you omitted the iodine (mordant) step in the procedure, what colors would the bacteria on your slide appear to be? ► The gram-positive bacteria would be purple, and the gram-negative would be pink All of the bacteria would look purple All of the bacteria would look pink The bacteria would not be stained at all (clear)
Out of the three gram-positive bacteria (Micrococcus luteus, Staphylococcus aureus, and Staphylococcus epidermidis). Which of the following test(s) will help me identify the unknown gram-positive bacteria? Gram Stain Wet Mount (motility testing) Endospore Staining Acid Fast Staining Differential & Selective Media (MacConkey, PEA, EMB, MSA Agar, Blood Agar, etc.) Oxidative-Fermentative Test Carbohydrate Fermentation MR-VP Testing Indole Production Citrate Utilization Triple Sugar Iron Testing Catalase Test
Out of all these tests, which 2 would help further understand this unknown lab. (Gram Stain and Growth Result is already done) Possible Tests (pick 2 that would help know the unknown bacteria) Gram stain morphology & arrangement color and growth characteristics on agar 37C, 30C and 25C (room temp) CAMP test starch hydrolysis test bacitracin susceptibility test glucose, lactose, sucrose, maltose fermentation tests methyl red/MR test Mannitol MacConkey Phenol Red Vogues-Proskauer/VP test citrate utilization test urea hydrolysis test phenylalanine deaminase test SIM test (Sulfide/Indole/Motility) Nitrate reduction test Oxidase test Catalase test
Table 1. Different Staining Techniques Gram Stain Acid Fast Endospore Capsule Flagella 1. Principle 2. Characteristic of Bacteria viewed using this stain. (e.g. bacterial type, strain, or targeting particular bacterial feature/s) 3. Stain/s Used 4. Type of Stain 5. Mechanism of Staining stain used) (of each 6. Flow of Method